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Series GSE66673 Query DataSets for GSE66673
Status Public on Mar 10, 2015
Title Identification of genes regulated during passaging of human osteosarcoma cell lines
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To identify the genes that are regulated during passaging of the parental osteosarcoma cells SAOS-2 and HOS with low metastatic potential, and SAOS-2-derived LM5 cells and Ki-ras transformed HOS cells (143B) with increased metastatic potential, we performed global gene expression profiling in early and late passage cells.
Overall design Total RNA was extracted from early and late passage cells cultured in three independent replicates for each condition. Cy3-labeled cRNA was synthesized and hybridized to Agilent human 8 x 60K microarrays. Raw data, obtained after array image analysis were normalized and used to retrieve differentially expressed genes in early and late passage cells.
Contributor(s) Muff R, Rath P, Ram Kumar RM, Lecca MR, Husmann K, Born W, Baudis M, Fuchs B
Citation(s) 25992885
Submission date Mar 09, 2015
Last update date Jun 10, 2019
Contact name Ram Mohan Ram Kumar
Organization name University Hospital Balgrist, University of Zurich, Zurich, Switzerland
Department Orthopaedics
Street address Forchstrasse 340
City Zurich
ZIP/Postal code 8008
Country Switzerland
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (24)
GSM1627704 143B_06_1
GSM1627705 143B_06_2_8
GSM1627706 143B_06_3
This SubSeries is part of SuperSeries:
GSE66675 Identification of genes regulated during passaging of human and mouse osteosarcoma cell lines
BioProject PRJNA277710

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE66673_Normalized_data_with_probename.txt.gz 4.0 Mb (ftp)(http) TXT
GSE66673_RAW.tar 73.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data are available on Series record

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