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| Status |
Public on Mar 10, 2015 |
| Title |
Identification of genes regulated during passaging of human osteosarcoma cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To identify the genes that are regulated during passaging of the parental osteosarcoma cells SAOS-2 and HOS with low metastatic potential, and SAOS-2-derived LM5 cells and Ki-ras transformed HOS cells (143B) with increased metastatic potential, we performed global gene expression profiling in early and late passage cells.
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| Overall design |
Total RNA was extracted from early and late passage cells cultured in three independent replicates for each condition. Cy3-labeled cRNA was synthesized and hybridized to Agilent human 8 x 60K microarrays. Raw data, obtained after array image analysis were normalized and used to retrieve differentially expressed genes in early and late passage cells.
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| Contributor(s) |
Muff R, Rath P, Ram Kumar RM, Lecca MR, Husmann K, Born W, Baudis M, Fuchs B |
| Citation(s) |
25992885 |
| Submission date |
Mar 09, 2015 |
| Last update date |
Jun 10, 2019 |
| Contact name |
Ram Mohan Ram Kumar |
| E-mail(s) |
rkumar@research.balgrist.ch
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| Organization name |
University Hospital Balgrist, University of Zurich, Zurich, Switzerland
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| Department |
Orthopaedics
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| Street address |
Forchstrasse 340
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| City |
Zurich |
| ZIP/Postal code |
8008 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE66675 |
Identification of genes regulated during passaging of human and mouse osteosarcoma cell lines |
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| Relations |
| BioProject |
PRJNA277710 |
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