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Series GSE66629 Query DataSets for GSE66629
Status Public on Oct 12, 2015
Title Hira is essential for normal transcriptional regulation and efficient de novo DNA methylation during mouse oogenesis
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary The integrity of chromatin, which provides a dynamic template for all DNA related processes in eukaryotes, is maintained through replication dependent and independent assembly pathways. To address the role of replication independent histone deposition, we deleted the histone H3.3 chaperone Hira in developing mouse oocytes. We show that chromatin of non-replicative developing oocytes is highly dynamic, and that lack of continuous H3.3/H4 deposition alters chromatin structure, resulting in increased DNase I sensitivity, the accumulation of DNA damage, and, ultimately, a severe fertility phenotype. On the molecular level, abnormal chromatin structure leads to a dramatic decrease in the dynamic range of gene expression, the appearance of spurious transcripts, and inefficient de novo DNA methylation. In contrast to the only minor transcriptional phenotype observed in mouse pluripotent cells, we unequivocally show the importance of histone replacement and chromatin homeostasis for transcriptional regulation and normal developmental progression in an in vivo context.
Overall design DNA methylome using BS-Seq of Hira wild-type and Hira deleted MII oocytes
Contributor(s) Nashun B, Hill PW, Smallwood SA, Dharmalingam G, Amouroux R, Clark SJ, Sharma V, Ndjetehe E, Pelczar P, Festenstein R, Kelsey G, Hajkova P
Citation(s) 26549683
Submission date Mar 06, 2015
Last update date May 15, 2019
Contact name Gavin Kelsey
Organization name The Babraham Institute
Street address Babraham Research Campus
City Cambridge
ZIP/Postal code CB22 3AT
Country United Kingdom
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (28)
GSM1626599 MII_WT-1
GSM1626600 MII_WT-2
GSM1626601 MII_WT-3
BioProject PRJNA277485
SRA SRP055927

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE66629_RAW.tar 675.3 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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