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Series GSE66602 Query DataSets for GSE66602
Status Public on Dec 01, 2015
Title The mRNA expression of FaDu-Ctrl, FaDu-ARID3B cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Microarray analyses for the identification of differences in gene expression patterns have increased our understanding of the molecular mechanism of ARID3B in HNSCC.We used gene expression analysis data from FaDu-ARID3B and FaDu-pCDH to identify differentially expressed probes.
The expression of the embryonic stem cell (ESC) signature in cancer cells indicates the coordinated regulation of the stemness genes in cancer stem cells, which are responsible for cancer initiation and dissemination. let-7 family microRNAs are crucial regulators for stem cell differentiation. In cancer cells, let-7 suppresses cancer stemness through targeting different oncogenes such as c-Myc, RAS, and HMGA2. However, most let-7 target genes are oncogenes rather than stemness factors, and the mechanism of let-7-repressed stemness is unclear. Here we demonstrate that let-7 supresses the formation of AT-rich interacting domain 3B (ARID3B) complex through targeting the expression of ARID3B, the interacting partner ARID3A, and importin 9. ARID3B complex recruits histone demethylase 4C (KDM4C) to the regulatory region of stemness genes for reducing histone 3 lysine 9 trimethylation, resulting in an open configuration of the chromatin of stemness genes. In cancer tissues, ARID3B expression correlates with the nuclear ARID3A expression and a worse prognosis. This result highlights the role of let-7 in regulating stemness through histone modifications.
Overall design The plasmids pCMVΔR8.9, pDVsVg (from the National RNAi Core Facility, Taiwan) and expression lentivectors (pCDH-GFP, pCDH-ARID3B) were co-transfected into 293T cells for virus packaging. The virus-containing supernatant was collected at 48 and 72 hours after transfection. While virus transduction, FaDu cells were infected with 10-fold virus concentrates supplemented with 8 μg/ml polybrene. pCDH-GFP as vector control.
Contributor(s) Muh-Hwa Y, Tsai-Tsen L
Citation(s) 26776511
Submission date Mar 06, 2015
Last update date Apr 23, 2018
Contact name Tsai-Tsen Liao
Phone +886-228235870
Organization name Institute of Clinical Medicine
Lab Dr. M.H. Yang's Lab
Street address No.201, Sec.2, Shih-Pai Rd. Peitou, Taipei, Taiwan, 11221, R.O.C
City Taipei
State/province State...
ZIP/Postal code 11221
Country Taiwan
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (2)
GSM1626092 18740_FD-pCDH.txt:gProcessedSignal(normalized)
GSM1626093 18738_FD-3B-1.txt:gProcessedSignal(normalized)
This SubSeries is part of SuperSeries:
GSE66603 let-7 controls cancer stemness through ARID3B
BioProject PRJNA277433

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE66602_RAW.tar 24.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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