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Series GSE66530 Query DataSets for GSE66530
Status Public on Mar 02, 2016
Title ChIP-seq validation of recombinant antibodies to histone post-translational modifications
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Recombinant antibodies to histone post-translational modifications (PTMs), with their essentially infinite renewability, could fundamentally eliminate a major source of low reproducibility in epigenetics research. Here, we report new recombinant antibodies to trimethylated Lys4 and Lys9, respectively, on histone H3. Quantitative characterization demonstrated their exquisite specificity and high affinity, and they performed well in common epigenetics applications, including ChIP. These results demonstrate the feasibility of generating recombinant antibodies to a range of histone marks, which will accelerate epigenetics research.
Overall design We characterized recombinant antibodies with native ChIP using HEK293 cells followed by deep sequencing.
Contributor(s) Hattori T, Grzybowski AT, Ruthenburg AJ, Koide S
Citation(s) 26862167
Submission date Mar 04, 2015
Last update date May 15, 2019
Contact name Takamitsu Hattori
Organization name The University of Chicago
Department Department of Biochemistry and Molecular Biology
Lab Koide lab
Street address 900 E. 57th Street
City Chicago
State/province IL
ZIP/Postal code 60637
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (10)
GSM1624500 Input for 309M3-B and 309M3-A
GSM1624501 309M3-B lot1_ChIPSeq experiment 1
GSM1624502 309M3-B lot1_ChIPSeq experiment 2
BioProject PRJNA277206
SRA SRP055826

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Supplementary file Size Download File type/resource
GSE66530_RAW.tar 3.8 Gb (http)(custom) TAR (of BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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