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Series GSE66351 Query DataSets for GSE66351
Status Public on Aug 12, 2018
Title DNA methylation profiling of neuron and glia for the dissection of cell type, age and Alzheimer’s disease-specific changes in the human brain
Organism Homo sapiens
Experiment type Methylation profiling by array
Summary Alzheimer’s disease (AD) is a progressive brain disorder caused by altered neuronal and glial cell functions. Illumina 450K profiling of cells (nuclei) sorted from post- mortem human brains allowed us assigning cell type- specific epigenetic changes to aging and AD progression. Among a few thousand cell type-specific differentially methylated CpGs (DMCGs) changing with age we identify prominent clusters in the AD genes, CLU, SYNJ2, ANK1 and MCF2L. A distinct set of age independent AD-specific DMCGs shows strong cell specific changes in the genes TOLLIP, BACE2, PKAR1B, PDE9A, ROBO2, FERMT2, UGT8, SPON1, INPP5D, LNFG and SRPK2. Some AD-specific DMCGs even reveal incremental changes with advancing BRAAK stages. In summary we observe a striking presence of DMCGs in major LOAD genes establishing novel connections between genetic and epigenetic contributions to AD. In addition the cell-specific association allows to functionally discriminate epigenetic effects of aging from those related to AD in the human brain.
Overall design Bulk and cell sorted post-mortem frontal cortex samples
Contributor(s) Gasparoni G, Bultmann S, Lutsik P, Kraus T, Vlcek J, Dietinger V, Steinmaurer M, Riemenschneider M, Kretzschmar HA, Giehse A, Leonhardt H, Walter J
Citation(s) 30045751
Submission date Feb 26, 2015
Last update date Jul 25, 2021
Contact name Pavlo Lutsik
Phone 04906813022881
Organization name Saarland University
Lab EpiGenetics
Street address Campus A2.4, 0.04
City Saarbruecken
ZIP/Postal code 66123
Country Germany
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (190)
GSM2808875 Dnr01_F_78_AD_Glia
GSM2808876 Dnr01_F_78_AD_Neuron
GSM2808877 Dnr02_M_76_AD_Glia
BioProject PRJNA276585

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE66351_RAW.tar 1.7 Gb (http)(custom) TAR (of IDAT)
Processed data included within Sample table

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