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Series GSE66345 Query DataSets for GSE66345
Status Public on Feb 27, 2015
Title A Circult Mechanism for Differentiating Positive and Negative Associations
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The ability to differentiate stimuli predicting positive or negative outcomes is critical for survival, and perturbations of emotional processing underlie many psychiatric disease states. Different neuronal populations of the basolateral amygdala complex (BLA) encode fearful or rewarding associations, but the molecular identity of these functionally distinct populations of BLA neurons remained unknown. Here, we show that BLA neurons projecting to the nucleus accumbens (NAc-projectors) or the centromedial amygdala (CeM-projectors) underwent opposing synaptic changes following fear or reward conditioning. The photostimulation of NAc projectors supported positive reinforcement while photostimulation of CeM projectors mediated negative reinforcement. In search of defining molecular characteristics of these functionally-distinct BLA neuronal populations, we compared gene expression profiles of NAc- and CeM-projectors.
Overall design For comparison of gene expression profiles of NAc- and CeM-projectors, we conducted two independent RNA sequencing experiments. In experiment-1, a total of n=9 samples (n=4 NAc- and n=5 CeM-projectors) are analyzed. In experiment-2, a total of n=8 samples (n=4 NAc- and n=4 CeM-projectors) are analyzed.
Contributor(s) Yorozu S, Gray JM
Citation(s) 25925480
Submission date Feb 26, 2015
Last update date May 15, 2019
Contact name Suzuko Yorozu
Organization name Harvard Medical School
Department Genetics
Lab Jesse Gray's lab
Street address 77 Avenue Louis Pasteur
City Boston
State/province Massachusettes
ZIP/Postal code 02115
Country USA
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (17)
GSM1620040 NAc projector, experiment-1: NAc_666
GSM1620041 NAc projector, experiment-1: NAc_671
GSM1620042 NAc projector, experiment-1: NAc_703
BioProject PRJNA276574
SRA SRP055558

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Supplementary file Size Download File type/resource
GSE66345_Summary_HTSeq_output_1stEX.txt.gz 304.2 Kb (ftp)(http) TXT
GSE66345_Summary_HTSeq_output_2ndEX.txt.gz 284.7 Kb (ftp)(http) TXT
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