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Series GSE66202 Query DataSets for GSE66202
Status Public on Dec 23, 2015
Title Intrinsic age-dependent changes and cell-cell contacts regulate nephron progenitor lifespan
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: To use single-cell RNA-Seq analysis of nephron progenitors in order to determine transcrptional differences as nephron progenitors age.
Methods: Using a combination of FACS sorting and a Fluidigm Single-cell auto-prep system, we generated high-throughput RNA-SEQ data of nephron progenitors during development
Results: Single cells transcriptome profiling of nephron progenitors revealed progressive age-dependent changes with heterogeneity increasing in older populations.
Overall design 96-single cell transcriptomes were determined from nephron progenitors of e14.5, e18.5 and P0 using Cited1GFP transgenic animals
Contributor(s) Chen S, Kopan R, Brunskill EW
Citation(s) 26460946
Submission date Feb 23, 2015
Last update date May 15, 2019
Contact name Raphael Kopan
Organization name Cincinnati Children's Hospital
Department Developmental Biology
Lab Kopan Lab
Street address 3333 Burnet Avenue
City Cincinnati
State/province Ohio
ZIP/Postal code 45011
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (91)
GSM1616785 Cell3
GSM1616786 Cell7
GSM1616787 Cell11
BioProject PRJNA276103
SRA SRP055425

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE66202_RAW.tar 64.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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