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Series GSE66189 Query DataSets for GSE66189
Status Public on Oct 01, 2015
Title Global Transcriptional Repression: Initial and Essential Step for Plasmodium Sexual Development [ChIP-seq]
Organism Plasmodium berghei ANKA
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Gametocytes are nonreplicative sexual forms that mediate malaria transmission to a mosquito vector. They are generated from asexual blood stage parasites, which proliferate in the circulation. However, it remains largely unknown as to how this transition is genetically regulated. Here, we report that an Apetala2 (AP2) family transcription factor, AP2-G2, regulates the transition as a transcriptional repressor. Disruption of AP2-G2 in the rodent malaria parasites, Plasmodium berghei, did not prevent commitment to the sexual stage but halted their development before manifesting sex-specific morphologies. ChIP-seq analysis revealed that AP2-G2 targets approximately 1,500 genes and recognizes a five-base motif on their promoters. Most of these target genes are required for asexual proliferation in the blood by the parasites, thereby suggesting that AP2-G2 blocks the program for asexual replication of parasites in the blood. DNA microarray analysis showed that the identified targets constituted approximately 70% of the upregulated genes in AP2-G2-depleted parasites, and a promoter assay using a centromere plasmid demonstrated that the binding motif functions as a cis-acting negative regulatory element. These results suggest that global transcriptional repression, which occurs during the initial phase of gametocytogenesis, is an essential step to promote conversion to the sexual stage.
 
Overall design Balb/c mice were infected with P. berghei expressing GFP-fused AP2-G2. Sulfadiazine was added to their drinking water in order to deplete asexual blood-stage parasites. Chromatins associated with GFP-tagged AP2-G2 was IPed with anti-GFP antibody, and.target genes of this transcription factor were predicted.
 
Contributor(s) Yuda M, kaneko i, iwanaga s, kato t
Citation(s) 26417110
Submission date Feb 23, 2015
Last update date May 15, 2019
Contact name masao yuda
E-mail(s) m-yuda@doc.medic.mie-u.ac.jp
Organization name mie university
Street address edobashi 2-174
City tsu
State/province mie
ZIP/Postal code 514-0001
Country Japan
 
Platforms (1)
GPL19817 AB 5500 Genetic Analyzer (Plasmodium berghei ANKA)
Samples (4)
GSM1616693 AP2-G2 ChIP-seq experiment-1 IP
GSM1616694 AP2-G2 ChIP-seq experiment-1 INPUT
GSM1616695 AP2-G2 ChIP-seq experiment-2 IP
This SubSeries is part of SuperSeries:
GSE66190 Global Transcriptional Repression: Initial and Essential Step for Plasmodium Sexual Development
Relations
BioProject PRJNA276082
SRA SRP055418

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Supplementary file Size Download File type/resource
GSE66189_RAW.tar 66.2 Mb (http)(custom) TAR (of BEDGRAPH, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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