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Series GSE66127 Query DataSets for GSE66127
Status Public on Feb 21, 2015
Title The Effects of Nanog HD Mutants on Mouse Embryonic Stem Cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We examined global gene expression patterns of mouse embryonic stem cells overexpressing EGFP, Nanog wild-type, or Nanog L122A mutants in normal, "-LIF" or "+RA" culture conditions by RNA-seq experiments. In “+RA” culture conditions, the expression patterns of the RA-responsive genes were slightly different in WT transfectants and more different in L122A transfectants compared with EGFP transfectants. These results suggested that L122A transfectants showed partial resistant activity against RA-induced differentiation, which was not found in WT mNANOG transfectants.
Overall design Examination of 3 different transfectants from mouse embryonic stem cell, RF8 line, in 3 different culture conditions.
Contributor(s) Hayashi Y, Yamanaka S
Citation(s) 25825768
Submission date Feb 20, 2015
Last update date May 15, 2019
Contact name Gladstone Bioinformatics
Organization name Gladstone Institutes
Department Data Science and Biotechnology
Lab Bioinformatics Core
Street address 1650 Owens St
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (9)
BioProject PRJNA275930
SRA SRP055200

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Supplementary file Size Download File type/resource
GSE66127_YH-288_FPKM_and_Counts_July_2_2014_mouse_mm9.txt.gz 2.0 Mb (ftp)(http) TXT
GSE66127_YH-288_FPKM_and_Counts_July_2_2014_mouse_mm9.xls.gz 4.8 Mb (ftp)(http) XLS
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