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Series GSE66023 Query DataSets for GSE66023
Status Public on Mar 12, 2016
Title Histone H3 globular domain acetylation identifies new class of enhancers
Organisms Homo sapiens; Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We report the acetylation of lysine residues in the globular domain of H3 (H3K64ac and H3K122ac) marks active gene promoters and also a subset of active enhancers in mouse embryonic stem cells (mESCs), human erythroleukemic cell line (K562). Moreover, we find a novel class of active functional enhancers in ESCs that are marked by H3K122ac but which lack H3K27ac. This work suggests that a more complex analysis of histone acetylation is required to identify enhancers than was previously considered.
Overall design Examination of histone modifications in mouse ESCs (2 biological replicates) and K562 cells
Contributor(s) Madapura PM, Grimes G, Bickmore WA
Citation(s) 27089178
Submission date Feb 18, 2015
Last update date May 15, 2019
Contact name Pradeepa M Madapura
Organization name Queen Mary University of London
Lab Pradeepa Madapura
Street address 4 Newark Street, Whitechapel
City London
State/province UK
ZIP/Postal code E1 2AT
Country United Kingdom
Platforms (3)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (10)
GSM2054689 46c_H3K122ac_rep1
GSM2054690 46c_H3K122ac_rep2
GSM2054691 46c_H3K64ac_rep1
Affiliated with GSE43103
BioProject PRJNA275703
SRA SRP055121

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE66023_46C_H3K122_merge.merged.sorted-W200-G600-islands-summary-FDR0.01.bed.gz 2.9 Mb (ftp)(http) BED
GSE66023_46C_H3K122acmerged.10bpres.bedGraph.gz 81.6 Mb (ftp)(http) BEDGRAPH
GSE66023_46C_H3K27ac.merged.sorted-W200-G200-islands-summary-FDR0.01.bed.gz 3.8 Mb (ftp)(http) BED
GSE66023_46C_H3K27acmerged.10bpres.bedGraph.gz 52.1 Mb (ftp)(http) BEDGRAPH
GSE66023_46C_H3K64ac.merged.sorted-W200-G600-islands-summary-FDR0.01.bed.gz 1.8 Mb (ftp)(http) BED
GSE66023_46C_H3K64acmerged.10bpres.bedGraph.gz 88.3 Mb (ftp)(http) BEDGRAPH
GSE66023_RAW.tar 171.3 Mb (http)(custom) TAR (of BED, BEDGRAPH)
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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