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Series GSE65952 Query DataSets for GSE65952
Status Public on Feb 16, 2016
Title Transcriptome analysis of Advanced glycation end products treated ligament cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Ossification of the posterior longitudinal ligament (OPLL) is formed by heterogeneous ossification of posterior longitudinal ligament. The patho-mechanism of OPLL is still largely unknown. Recently, disorders of metabolism are thought to be the center of many diseases such as OPLL. Advanced glycation end product (AGE) are accumulated in many extracellular matrixes such as ligament fibers, and it can functions as cellular signal through its receptor (RAGE), contributing to various events such as atherosclerosis or oxidative stress. However, its role in OPLL formation is not yet known. Therefore, we performed high-through-put RNA sequencing on primary posterior longitudinal ligament cells treated with different doses of AGEs (1µM, 5µM and negative control), with or without BMP2 (1µM).
Overall design mRNA profiles of Primary human posterior longitudinal ligament cells stimulated with various stimuli (Control, 1µM AGE-BSA, 5µM AGE-BSA, 1µM AGE-BSA with BMP2, 5µM AGE-BSA with BMP2) were generated by deep sequencing on Ion Proton
Contributor(s) Xu C, He Z
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Submission date Feb 16, 2015
Last update date May 15, 2019
Contact name Chen Xu
Phone +86-13774294166
Organization name The Second Military Medical University
Street address No, 800, Rd. Xiangyin
City Shanghai
ZIP/Postal code 200433
Country China
Platforms (1)
GPL17303 Ion Torrent Proton (Homo sapiens)
Samples (5)
GSM1611656 BSA Control
GSM1611657 1µM AGE-BSA
GSM1611658 5µM AGE-BSA
BioProject PRJNA275546
SRA SRP055063

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Supplementary file Size Download File type/resource
GSE65952_All_Counts.txt.gz 354.4 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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