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Series GSE65791 Query DataSets for GSE65791
Status Public on Feb 10, 2017
Title Generation of PDGFRα+ Cardioblasts from Pluripotent Stem Cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary We uncover a novel class of cardiac lineage cells, PDGFRα+Flk1- cardioblasts (PCB), from mouse and human pluripotent stem cells (PSC) using CsAYTE, a combination of small molecules – Cyclosporin A (CsA), ROCK inhibitor Y27362, antioxidant Trolox, and ALK5 inhibitor EW7197.
To elucidate the genome-wide characteristics of PCB, microarray analysis was performed and compared with the spontaneously formed PCB, which were obtained without CsAYTE incubation (hereafter referred to as “sfPCB”), Flk1+ mesodermal precursor cells (MPC), and αMHC+ cardiomyocytes (M+CM)
 
Overall design Sorting of cells was performed by flow cytometry (Flk1+ MPC at day 4.5, sfPCB and PCB at day 5.5, and M+CM at day 10.5 from PSC differentiation) and microarray analysis was performed.
 
Contributor(s) Cho SW, Koh GY
Citation(s) 28165490
Submission date Feb 10, 2015
Last update date Nov 01, 2017
Contact name Gou Young Koh
E-mail(s) gykoh@kaist.ac.kr
Phone 82-42-350-2638
Organization name KAIST
Street address 291 Daehark-ro, Yuseong-gu
City Daejeon
ZIP/Postal code 305-701
Country South Korea
 
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (4)
GSM1606391 sfPCB
GSM1606392 PCB
GSM1606393 Flk1+MPC
Relations
BioProject PRJNA275062

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE65791_RAW.tar 8.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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