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Series GSE65658 Query DataSets for GSE65658
Status Public on Feb 06, 2015
Title Mouse sinoatrial node transcriptome
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary RNA Sequencing of Mouse Sinoatrial Node Reveals an Upstream Regulatory Role for Islet-1 in Cardiac Pacemaker Cells

Rationale: Treatment of sinus node disease with regenerative or cell-based therapies will require a detailed understanding of gene regulatory networks in cardiac pacemaker cells (PCs).

Objective: To characterize the transcriptome of PCs using RNA sequencing, and to identify transcriptional networks responsible for PC gene expression.

Methods and Results: We used laser capture micro-dissection (LCM) on a sinus node reporter mouse line to isolate RNA from PCs for RNA sequencing (RNA-Seq). Differential expression and network analysis identified novel SAN-enriched genes, and predicted that the transcription factor Islet-1 (Isl1) is active in developing pacemaker cells. RNA-Seq on SAN tissue lacking Isl1 established that Isl1 is an important transcriptional regulator within the developing SAN.

Conclusions: (1) The PC transcriptome diverges sharply from other cardiomyocytes; (2) Isl1 is a positive transcriptional regulator of the PC gene expression program.
Overall design There are 25 RNA-Sequencing Samples
Contributor(s) Vedantham V, Deo RC
Citation(s) 25623957
Submission date Feb 05, 2015
Last update date May 15, 2019
Contact name Vasanth Vedantham
Organization name University of California, San Francisco
Department Medicine-Cardiology
Street address 505 Parnassus Avenue, M1176D
City San Francisco
State/province CA
ZIP/Postal code 94143
Country USA
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (25)
GSM1602583 E14.5-RA-1
GSM1602584 E14.5-RA-2
GSM1602585 E14.5-RA-3
SRA SRP053173
BioProject PRJNA274622

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE65658_SAN_RA_Isl1_TMM.txt.gz 1.3 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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