GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE65621 Query DataSets for GSE65621
Status Public on May 27, 2015
Title Asymmetry of STAT action in driving IL-27 and IL-6 transcriptional outputs and cytokine specificity
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary IL-6 and IL-27 have antagonistic and overlapping functions, signal through a shared receptor subunit and employ the same downstream STAT proteins. To evaluate the degree of specificity and redundancy for these cytokines, we quantified global transcriptomic changes induced by the two cytokines and determined the relative contributions of STAT1 and STAT3 using genetic models and ChIP-seq. We found a high degree of overlap of the transcriptomes induced by IL-6 and IL-27 and extremely few examples in which the cytokines acted in opposition. Using STAT deficient cells and T cells from patients with gain-of-function STAT1 mutations, we show that STAT3 was responsible for the overall transcriptional output driven by both cytokines, whereas STAT1 was the driver of cytokine specificity. STAT1 did not compensate for the lack STAT3; on the contrary, much of STAT1 binding to chromatin was STAT3 dependent. Thus, STAT1 shapes the specific cytokine signature superimposed upon STAT3’s action.
Overall design Integrated analysis of transcriptome and transcription factor binding data from cytokine treated CD4+T cells
Contributor(s) Hirahara K, Sun H, Brooks SR, Gutierrez-Cruz G, Iwata S, Kanno Y, O'Shea JJ
Citation(s) 25992861
Submission date Feb 04, 2015
Last update date May 15, 2019
Contact name Yuka Kanno
Phone 301-402-3008
Organization name NIH
Department NIAMS
Street address 10 Center Drive, Rm13C120
City Bethesda
State/province MD
ZIP/Postal code 20892-1930
Country USA
Platforms (3)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (102)
GSM1601720 STAT1_WT_nc_1684
GSM1601721 STAT3_WT_nc_1685
GSM1601722 STAT1_WT_IL6_1686
BioProject PRJNA274489
SRA SRP053186

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE65621_RAW.tar 114.6 Mb (http)(custom) TAR (of BW)
GSE65621_table1_mouse_RPKM.xlsx.gz 10.1 Mb (ftp)(http) XLSX
GSE65621_table2_human_RPKM.xlsx.gz 5.7 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Processed data are available on Series record
Processed data provided as supplementary file
Raw data are available in SRA

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap