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Series GSE6559 Query DataSets for GSE6559
Status Public on Jan 02, 2007
Title Expression data from primary human keratinocytes
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Oncogenic Ras induces epidermal cell growth arrest. Induction of the JNK/Ap1 signaling cascade by expression of MKK7 overcomes Ras-induced cell growth arrest in a manner dependent on AP1 fucntion.
We used microarrays to detail the global programme of gene expression in response to MKK7, Ras activation and AP1 inhibition
Keywords: response to specific genetic change
Overall design To study the gene expression profile responsive to Ras and MKK7 expression. We used retroviral expression system to express LacZ, MKK7, Ras, DNc-Jun, MKK7+Ras, and MKK7+Ras +DNc-Jun in primary human keratinocytes, and then extract total RNA from the transduced cells for gene expression analysis.
Contributor(s) Zhang JY, Adams AA, Ricky TW, Tao S, Khavari PA
Citation(s) 17440097
Submission date Dec 18, 2006
Last update date Dec 06, 2018
Contact name Jennifer Yunyan Zhang
Phone 919-684-6794
Organization name Duke University
Department Dermatology
Lab Zhang
Street address 3 Genome Ct, rm4130
City Durham
State/province NC
ZIP/Postal code 27710
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (6)
GSM151731 Primary human keratinocytes transduced to express LacZ control
GSM151732 Primary human keratinocytes transduced to expressMKK7
GSM151733 Primary human keratinocytes transduced to express Ras
BioProject PRJNA98775

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Supplementary file Size Download File type/resource
GSE6559_RAW.tar 12.4 Mb (http)(custom) TAR (of CEL)

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