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| Status |
Public on Aug 10, 2015 |
| Title |
Analysis of the molecular dialogue between Botrytis cinerea and Vitis vinifera reveals a clear shift in defence mechanisms during berry ripening. |
| Platform organism |
Botrytis cinerea |
| Sample organism |
Botrytis cinerea B05.10 |
| Experiment type |
Expression profiling by array
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| Summary |
Full transcriptomes of the Botrytis cinerea wild-type strain B0510 inoculated on mature grapevine berries (Marselan cultivar) at 16h, 24h, 48h, and in vitro were compared to identify B. cinerea genes diffentially expressed during the infection stages.
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| Overall design |
Grapevine berries were inoculated with Botrytis cinerea conidia and samples were taken at 16h, 24h and 48 hours post-inoculation. An additional control sample corresponding to mycelium growing on static liquid minimal medium was included in the experimental design in order to identify genes that would be differentially expressed between in vitro and in planta growth conditions. 3-4 replicates were performed. The 13 total-RNA samples were used for hybridization on NimbleGen 4plex gene expression arrays (20,885 gene models from Botrytis cinerea with three 60-mer probes per gene).
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| Contributor(s) |
Kellioniemi J, Trouvelot S, Heloir MC, Simon A, Dalmais B, Frettinger P, Cimerman A, Fermaud M, Roudet J, Baulande S, Bruel C, Choquer M, Couvelard L, Duthieuw M, Ferrarini A, Flors V, Le Pecheur P, Loisel E, Morgant G, Poussereau N, Pradier JM, Rascle C, Trda L, Poinssot B, Viaud M |
| Citation(s) |
26267356 |
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| Submission date |
Feb 03, 2015 |
| Last update date |
Nov 16, 2015 |
| Contact name |
Adeline SIMON |
| Organization name |
INRAE
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| Lab |
BIOGER
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| Street address |
22 place de l'agronomie
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| City |
Palaiseau |
| ZIP/Postal code |
91120 |
| Country |
France |
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| Platforms (1) |
| GPL17773 |
NimbleGen - Botrytis cinerea - 4*72K - INRA improved (2009) [091123_INRA_Botrytis_expr] |
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| Samples (13)
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| Relations |
| BioProject |
PRJNA274369 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE65559_RAW.tar |
17.3 Mb |
(http)(custom) |
TAR (of PAIR) |
| Processed data included within Sample table |
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