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Series GSE65323 Query DataSets for GSE65323
Status Public on Aug 18, 2016
Title Genome wide extracellular signal-regulated kinase (ERK) pathway components interaction with human genome
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Several recent studies, including ours, show that most components of signal transduction cascades including the extracellular signal-regulated protein kinase (ERK), that once were thought to act predominantly in cytoplasm, are in fact recruited to chromatin and are integral components of transcriptional complexes. However, their distribution along whole genome remains uncovered. Here we investigate genome wide recruitment of the ERK pathway components using chromatin immunoprecipitations (ChIP) followed by deep sequencing, ChIP-Seq.
Overall design Hela-S3 cells were starved for 48 hours (control) and stimulated with EGF at concentration of 100ng/mL for 20 minutes. Cells were fixed with formaldehyde, chromatin isolated and subjected to ChIP reaction. Two technical replicates of ChIP reaction followed by sequencing were performed.
Contributor(s) Mikula M, Goryca K, Ledwon J, Skrzypczak M, Grzelak M, Dabrowska M, Kulecka M, Ginalski K, Bomsztyk K, Ostrowski J
Citation(s) 27587583
Submission date Jan 26, 2015
Last update date May 15, 2019
Contact name Krzysztof Goryca
Organization name Uniwersytet Warszawski
Department CeNT
Street address Banacha 2c
City Warszawa
ZIP/Postal code 02-097
Country Poland
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (20)
GSM1592485 13_Pol2_control_repl_1
GSM1592486 14_Pol2_control_repl_2
GSM1592487 15_Pol2_EGF20_repl_1
BioProject PRJNA273670
SRA SRP052844

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Supplementary file Size Download File type/resource
GSE65323_macs.Pol2-Egf.vs.Pol2.peaks.bed.gz 24.5 Kb (ftp)(http) BED
GSE65323_macs.pBRAF-EGF.vs.pBRAF.merged.peaks.bed.gz 8.3 Kb (ftp)(http) BED
GSE65323_macs.pEGFR-Egf.vs.pEGFR.merged.peaks.bed.gz 12.4 Kb (ftp)(http) BED
GSE65323_macs.pERK_Egf.vs.pERK.merged.peaks.bed.gz 33.4 Kb (ftp)(http) BED
GSE65323_macs.pMEK_Egf.vs.pMEK.merged.peaks.bed.gz 6.9 Kb (ftp)(http) BED
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