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Series GSE65233 Query DataSets for GSE65233
Status Public on Aug 01, 2015
Title The effect of THADA knockdown on Huh7 cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We use lentivirus carrying shRNA for THADA to evaluate the efect of THADA knockdown in Huh7 cells.
 
Overall design The pGIPZ shRNA plasmid specific to human THADA (clone ID V3LHS_318037 Open Biosystems, Inc.) or the pGIPZ non-silencing shRNAmir lentiviral plasmid (as a control) was cotransfected with the pPACKH1 plasmid mix in HEK 293 cells. Stable clones of Huh7 cells with THADA knockdown were routinely maintained in a medium containing of puromycin (500ng/ml)
 
Contributor(s) Yoshida T
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Submission date Jan 23, 2015
Last update date Jan 09, 2018
Contact name Takafumi Yoshida
E-mail(s) tayoshi@med.kurume-u.ac.jp
Organization name Kurume University
Department Department of Medicine
Lab Division of Gastroenterology
Street address 67, asahi-machi
City Kurume
State/province Fukuoka
ZIP/Postal code 830-0011
Country Japan
 
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (2)
GSM1590392 KD
GSM1590393 control
Relations
BioProject PRJNA273458

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE65233_RAW.tar 24.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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