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Series GSE65201 Query DataSets for GSE65201
Status Public on Jan 23, 2015
Title CDK7-dependent Transcriptional Addition in Basal-like Breast Cancer (ChIP-Seq)
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Basal-like breast cancer (BBC) is a highly aggressive form of breast cancer that exhibits extremely high levels of genetic complexity and yet a relatively uniform transcriptional program. We postulate that BBC might be highly dependent on uninterrupted transcription of a key set of genes within this gene expression program and might therefore be exceptionally sensitive to inhibitors of transcription. Utilizing a novel kinase inhibitor and CRISPR/Cas9-mediated gene editing, we show here that basal but not luminal breast cancer cells are exceptionally dependent on CDK7, a transcriptional cyclin-dependent kinase. BBC cells are unique in their dependence on this transcriptional CDK and suffer apoptotic cell death upon CDK7 inhibition. An “Achilles cluster” of BBC-specific genes are extremely sensitive to CDK7 inhibition and frequently associated with super-enhancers. We conclude that CDK7 mediates transcriptional addiction to a vital cluster of genes in BBC and CDK7 inhibition may be useful therapy for this challenging cancer.
Overall design ChIP-Seq for H3K27ac in basal-like breast cancer and luminal-like breast cancer cell lines
Contributor(s) Wang Y, Kwiatkowski N, Abraham BJ, Lee TI, Zhao J, Young RA, Gray NS
Citation(s) 26406377
Submission date Jan 22, 2015
Last update date May 15, 2019
Contact name Richard A Young
Phone 617-258-5219
Organization name Whitehead Institute for Biomedical Research
Lab Young Lab
Street address 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (8)
GSM1589470 MDA-MB-468 H3K27Ac [lab: Zhao-DFCI]
GSM1589471 MDA-MB-468 Input [lab: Zhao-DFCI]
GSM1589472 ZR-75-1 H3K27Ac [lab: Zhao-DFCI]
This SubSeries is part of SuperSeries:
GSE69107 CDK7-dependent Transcriptional Addiction in Basal-like Breast Cancer
BioProject PRJNA273393
SRA SRP052748

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Supplementary file Size Download File type/resource
GSE65201_RAW.tar 513.0 Mb (http)(custom) TAR (of WIG)
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Raw data are available in SRA
Processed data provided as supplementary file

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