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Series GSE65167 Query DataSets for GSE65167
Status Public on Mar 30, 2016
Title ChIP-seq analysis of Arid1a and C/ebpα in the mouse livers
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Arid1a is the subunit of SWI/SNF complex, which was reported to guide SWI/SNF to DNA. Here, we found that loss of Arid1a in the liver and other adult tissues results in improved organ regeneration. Within SWI/SNF complexes, Arid1a physically interacts with C/ebpα, a hepatocyte transcription factor that drives maturation and limits proliferation. Genome-wide analysis showed that loss of Arid1a reduces the recruitment and activity of C/ebpα on target promoters, resulting in expression programs that favor regeneration and cellular fitness during injury. Arid1a binding is enriched in promoters near transcriptional start sites (TSSs), and C/ebpα binds at precisely the same positions, indicating that Arid1a facilitates C/ebpα binding across the genome.
Overall design Perfuse and isolate primary hepatocytes from mice livers, analysis of genomic occupancy of C/ebpα and H3K4me2 in hepatocytes from Arid1a WT and Arid1a liver specific KO mice by ChIP-seq. Analysis of genomic occupancy of Arid1a in the hepatocytes from V5-Arid1a transgenic mouse by ChIP-seq.
Contributor(s) Sun X, Xing C, Zhu H
Citation(s) 27044474
Submission date Jan 21, 2015
Last update date May 15, 2019
Contact name Xuxu Sun
Organization name shanghai jiao tong university
Department school of medicine
Street address 227 south chongqing road
City shanghai
ZIP/Postal code 200025
Country China
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (21)
GSM1588476 cebpa-WT rep1
GSM1588477 cebpa-WT rep2
GSM1588478 cebpa-KO rep1
BioProject PRJNA273315
SRA SRP052719

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Supplementary file Size Download File type/resource
GSE65167_RAW.tar 1.2 Gb (http)(custom) TAR (of BED, BIGWIG, BROADPEAK)
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Raw data are available in SRA
Processed data provided as supplementary file

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