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Series GSE65138 Query DataSets for GSE65138
Status Public on Sep 25, 2015
Title Effect of cortistatin A (CA) on enhancer occupancy in CA-sensitive and -insensitive human cell lines
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We characterized the marine natural product cortistatin A (CA) as an inhibitor of CDK8 to determine whether pharmacologic inhibition of CDK8 regulates super-enhancer function and inhibits AML proliferation.
In this series, we use ChIP-seq of H3K27ac, H3K4me1, MED1, CDK8, BRD4, and RNA polymerase II (PolII) to examine enhancer occupancy and transcriptional response to CA in human cells lines.
Overall design ChIP-seq was carried out in CA-sensitive: MOLM-14 cells (H3K27Ac, H3K4me1, MED1, CDK8, BRD4, and PolII), MV4;11(H3K27Ac, CDK8), SET2 (H3K27Ac, CDK8), and in CA-insensitive cells: K562 (CDK8).
Contributor(s) Pelish HE, Liau BB, Nitulescu II, Lemieux ME, Shair MD
Citation(s) 26416749
Submission date Jan 21, 2015
Last update date May 15, 2019
Contact name Matthew D. Shair
Organization name Harvard University
Department Chemistry and Chemical Biology
Lab Shair
Street address 12 Oxford Street
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (53)
GSM1587891 MOLM14_input_untreated_1
GSM1587892 MOLM14_H3K4me1_untreated_1
GSM1587893 MOLM14_H3K27ac_untreated_1
This SubSeries is part of SuperSeries:
GSE65161 Mediator kinase inhibition further activates super-enhancer-associated genes in AML
BioProject PRJNA273301
SRA SRP052715

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Supplementary file Size Download File type/resource
GSE65138_MOLM14_BRD4_DMSO_IDR.bed.gz 104.5 Kb (ftp)(http) BED
GSE65138_MOLM14_BRD4_iBET_IDR.bed.gz 94.5 Kb (ftp)(http) BED
GSE65138_MOLM14_CDK8_DMSO_IDR.bed.gz 584.1 Kb (ftp)(http) BED
GSE65138_MOLM14_CDK8_iBET_IDR.bed.gz 249.3 Kb (ftp)(http) BED
GSE65138_MOLM14_PollII_traveling_ratios.xlsx 1.5 Mb (ftp)(http) XLSX
GSE65138_suppl_t3.xlsx 11.7 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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