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Series GSE65044 Query DataSets for GSE65044
Status Public on Jan 04, 2019
Title Characterization of resident CD8+ Trm cell populations using whole-genome biomarker analysis
Organism Mus musculus
Experiment type Expression profiling by array
Summary This study seeks a better understanding of the functional differences between naturally acquired murine CD8+ T resident memory (Trm) cells of three distinct mucosal environments; the small intestine, the lung and the liver. CD8+ Trm cells of healthy young adult mice were retrieved from their respective organ environments by automated organ disintegration and MACS sorting, and compared with each other by whole genome gene expression profiling. Peripheral blood derived circulating CD8+ Tem cells were also analyzed, serving as nonresident peripheral memory T cell controls, in an attempt to identify common characteristics of resident mucosal CD8+ Trm subsets, as well.
Overall design The livers, lungs, and small intestines of 8-16 weeks old healthy wild type C57Bl/6 mice were isolated to collect CD8+ T resident memory (Trm) cells by MACS cell sorting (n=5-6). Cirulating CD8+ T effector memory (Tem) cells were also collected from the peripheral blood by MACS sorting to serve as a reference, representing a circulating, nonresident CD8+ T memory T cell control. Finally, whole organ lysates were also collected as controls for possible parenchymal cell contamination of MACS sorted cells (n=3)
Contributor(s) Ersek B, Lupsa N, Pocza P, Sarzsinszky E, Toth A, Bagita B, Hegyesi H, Falus A, Safrany G, Andras M, Buzas E, Pos Z
Citation(s) 27137185
Submission date Jan 16, 2015
Last update date Jan 05, 2019
Contact name Nikolett Lupsa
Organization name Semmelweis University
Department Genetics, Cell-and Immunbiology
Lab MTA-SE Lendület Research Group
Street address Nagyvárad tér 4
City Budapest
State/province Pest
ZIP/Postal code 1085
Country Hungary
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (35)
GSM1586220 CD8+ Trm, liver 1
GSM1586221 CD8+ Trm, liver 2
GSM1586222 CD8+ Trm, liver 3
This SubSeries is part of SuperSeries:
GSE65045 Comparative analysis of murine CD8+ resident memory and effector T cells in various organ environments
BioProject PRJNA272783

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE65044_RAW.tar 77.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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