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Series GSE64959 Query DataSets for GSE64959
Status Public on Jan 27, 2015
Title RNA-SEQ analysis of various developing and adult kidney components
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We used micro-dissection techniques and/or FACS to isolate cell types from the developing and adult kidney (E11.5 ureteric buds, E12.5, P1 and P4 cap mesenchyme, E15.5 collecting ducts, proximal tubules, ureter, Adult renal proximal tubules, podocytes, endothelial and mesangial cells). RNA-SEQ analysis was performed to determine the transcriptional profile of each cell type, identify component specific transcripts and isoforms and cell-type specific long-noncoding RNAs. In addition the unbiased nature of RNA-SEQ will potentially identify novel transcripts that have not been annotated in the database.
Overall design Total RNA is obtained from micro-dissected and/or FACS isolated embryonic and adult kidney components. The long term goal is to generate a transcriptional atlas of developing kidney.
Web link
Contributor(s) Potter A, Brunskill EW, Potter S
Citation(s) 25053437
Submission date Jan 14, 2015
Last update date May 15, 2019
Contact name GUDMAP Developers
Phone +44 131 651 8500
Organization name IGMM MRC Human Genetics Unit
Lab GUDMAP Database Group
Street address Crewe Road
City Edinburgh
ZIP/Postal code EH4 2XU
Country United Kingdom
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (20)
GSM1585028 Crym P1 cDNA - Nugen
GSM1585029 Crym P4 cDNA - Nugen
GSM1585030 Adult Proximal Tubules - 1
BioProject PRJNA272600
SRA SRP052230

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE64959_RPKM_Gudmap_Revised_11_19_14a.txt.gz 2.3 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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