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Series GSE64877 Query DataSets for GSE64877
Status Public on Jun 22, 2016
Title Global re-wiring of p53 transcription regulation by the hepatitis B virus X (HBx) protein [ChIP-Seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary As HBx has been reported to interact with p53 and alter the recruitment of p53 to its binding sites, we obtained a comprehensive genome-wide profile of deregulated p53 transcription complex-DNA binding by the HBx protein using massively parallel deep sequencing coupled to p53 chromatin immunoprecipitation (ChIP-Seq) on HBx-expressing and control HepG2 liver cell culture model system.
Overall design HepG2 cells were transduced with recombinant HBx and control adenoviruses 24 hours post seeding. HepG2 cells were treated with UVC (254 nm) irradiation 48 hours postransduction and harvested 24 hours after UV irradiation. 30 µg of control and HBx-expressing UV-treated HepG2 cell lysates were immunoprecipitated using 4 µg of p53 FL-393 antibody (Santa Cruz Biotechnology). The subsequent yield and size of the ChIP library generated was quantified, followed by sequencing using HiSeq (Illumina, CA, USA) to read counts of 7.7 M total reads.
Contributor(s) Chan C, Thurnherr T, Lee C
Citation(s) 27302019
Submission date Jan 12, 2015
Last update date May 15, 2019
Contact name Thomas Thurnherr
Organization name NCCS
Street address 11 Hospital Drive
City Singapore
ZIP/Postal code 169610
Country Singapore
Platforms (1)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
Samples (2)
GSM1581946 HepG2 control detection of p53 binding sites
GSM1581947 HepG2 treated detection of p53 binding sites
This SubSeries is part of SuperSeries:
GSE64878 Global re-wiring of p53 transcription regulation by the hepatitis B virus X (HBx) protein
BioProject PRJNA272433
SRA SRP052031

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Supplementary file Size Download File type/resource
GSE64877_RAW.tar 310.0 Kb (http)(custom) TAR (of BED)
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Raw data are available in SRA
Processed data provided as supplementary file

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