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| Status |
Public on Jan 10, 2015 |
| Title |
Peripheral regions and central regions from HT-29 tumors |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Gene expression of peripheral regions and central regions from HT-29 tumors
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| Overall design |
Sample tissues were immediately frozen by liquid nitrogen after isolation. Total RNAs were extracted from samples with a PureLink RNA Mini Kit (Life Technologies). The integrity of total RNAs was evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Low Input Quick Amp Labeling Kit, one-color (Agilent Technologies) was used to prepare Cy3-labelled target cRNA according to the manufacturer's instructions. Labeled cRNAs were hybridized with a SurePrint G3 Human GE 8×60K Microarrays (Agilent Technologies). Three separate hybridizations were performed for each sample. Array images were captured using a DNA Microarray Scanner (Agilent Technologies), and data were analyzed using Feature Extraction Software (Agilent Technologies) to obtain background-corrected signal intensities. Data were further analyzed with GeneSpring GX Software (Version 11.0, Agilent Technologies). After data filtering, mRNAs differentially expressed in target cells versus controls were assessed by Fisher’s exact test, followed by multiple corrections using the Benjamini and Hochberg false discovery rate (FDR) method. Gene sets with a FDR q-value < 0.05 were considered to be significant.
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| Contributor(s) |
Yoshii Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 09, 2015 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Yukie Yoshii |
| E-mail(s) |
yoshii.yukie@qst.go.jp
|
| Phone |
043-206-3429
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| Organization name |
National Institute of Radiological Sciences
|
| Street address |
Anagawa 4-9-1, Inage
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| City |
Chiba |
| ZIP/Postal code |
263-8555 |
| Country |
Japan |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA272132 |
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