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Series GSE64831 Query DataSets for GSE64831
Status Public on Jan 10, 2015
Title Peripheral regions and central regions from HT-29 tumors
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Gene expression of peripheral regions and central regions from HT-29 tumors
Overall design Sample tissues were immediately frozen by liquid nitrogen after isolation. Total RNAs were extracted from samples with a PureLink RNA Mini Kit (Life Technologies). The integrity of total RNAs was evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Low Input Quick Amp Labeling Kit, one-color (Agilent Technologies) was used to prepare Cy3-labelled target cRNA according to the manufacturer's instructions. Labeled cRNAs were hybridized with a SurePrint G3 Human GE 8×60K Microarrays (Agilent Technologies). Three separate hybridizations were performed for each sample. Array images were captured using a DNA Microarray Scanner (Agilent Technologies), and data were analyzed using Feature Extraction Software (Agilent Technologies) to obtain background-corrected signal intensities. Data were further analyzed with GeneSpring GX Software (Version 11.0, Agilent Technologies). After data filtering, mRNAs differentially expressed in target cells versus controls were assessed by Fisher’s exact test, followed by multiple corrections using the Benjamini and Hochberg false discovery rate (FDR) method. Gene sets with a FDR q-value < 0.05 were considered to be significant.
Contributor(s) Yoshii Y
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Submission date Jan 09, 2015
Last update date Nov 27, 2018
Contact name Yukie Yoshii
Phone 043-206-3429
Organization name National Institute of Radiological Sciences
Street address Anagawa 4-9-1, Inage
City Chiba
ZIP/Postal code 263-8555
Country Japan
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (6)
GSM1581337 Central_rep1
GSM1581338 Central_rep2
GSM1581339 Central_rep3
BioProject PRJNA272132

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE64831_AR1017_Change.txt.gz 57.4 Kb (ftp)(http) TXT
GSE64831_AR1017_Ratio.txt.gz 9.7 Mb (ftp)(http) TXT
GSE64831_AR1017_Signal.txt.gz 9.3 Mb (ftp)(http) TXT
GSE64831_RAW.tar 23.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data are available on Series record

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