|Public on Apr 01, 2015
|Specific gene expression related to epiblast development
|Expression profiling by array
|To explore genes involved in epiblast development, we have employed whole genome microarray expression profiling as a discovery platform to identify genes. Embryoid body (EB) formation induces embryonic stem (ES) cells to differentiate into germ layers via epiblasts and extra-embryonic tissues, whereas ES cells give rise only to extra-embryonic tissues in monolayer culture. We compared gene expression profiles of ES cells in each differentiation culture methods at day 1-3 or 4 of differentiation. The expression of organizer related genes (Lhx1, Cer1 and Foxa2) was specifically induced at day 3 of EBs, but not in monolayer culture. In epiblast model cells, the overexpression of Lhx1 induced gene expression of organizer and meso-endodermal markers, suggested that Lhx1 was related to epiblast differentiation.
|Gene expression related epiblast development was measured at undifferentiated state and day 1-3 or 4 of differentiation. Two independent experiments were performed at each time using different people for each experiment.
|Ikeda N, Hisatome I, Shirayoshi Y
|Jan 07, 2015
|Last update date
|Jan 12, 2017
|Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science,Tottori University
|Department of Genetic Medicine and Regenerative Therapeutics
|Division of Regenerative Medicine and Therapeutics
|Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)