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Series GSE64715 Query DataSets for GSE64715
Status Public on Apr 01, 2015
Title Specific gene expression related to epiblast development
Organism Mus musculus
Experiment type Expression profiling by array
Summary To explore genes involved in epiblast development, we have employed whole genome microarray expression profiling as a discovery platform to identify genes. Embryoid body (EB) formation induces embryonic stem (ES) cells to differentiate into germ layers via epiblasts and extra-embryonic tissues, whereas ES cells give rise only to extra-embryonic tissues in monolayer culture. We compared gene expression profiles of ES cells in each differentiation culture methods at day 1-3 or 4 of differentiation. The expression of organizer related genes (Lhx1, Cer1 and Foxa2) was specifically induced at day 3 of EBs, but not in monolayer culture. In epiblast model cells, the overexpression of Lhx1 induced gene expression of organizer and meso-endodermal markers, suggested that Lhx1 was related to epiblast differentiation.
Overall design Gene expression related epiblast development was measured at undifferentiated state and day 1-3 or 4 of differentiation. Two independent experiments were performed at each time using different people for each experiment.
Contributor(s) Ikeda N, Hisatome I, Shirayoshi Y
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Submission date Jan 07, 2015
Last update date Jan 12, 2017
Contact name Akira Hasegawa
Organization name Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science,Tottori University
Department Department of Genetic Medicine and Regenerative Therapeutics
Lab Division of Regenerative Medicine and Therapeutics
Street address 36-1, Nishimachi
City Yonago
State/province Tottori
ZIP/Postal code 683-8503
Country Japan
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (12)
GSM1577775 LIF-1
GSM1577776 LIF-2
GSM1577777 EBd1-1
BioProject PRJNA271729

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE64715_RAW.tar 107.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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