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| Status |
Public on Apr 01, 2015 |
| Title |
Specific gene expression related to epiblast development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To explore genes involved in epiblast development, we have employed whole genome microarray expression profiling as a discovery platform to identify genes. Embryoid body (EB) formation induces embryonic stem (ES) cells to differentiate into germ layers via epiblasts and extra-embryonic tissues, whereas ES cells give rise only to extra-embryonic tissues in monolayer culture. We compared gene expression profiles of ES cells in each differentiation culture methods at day 1-3 or 4 of differentiation. The expression of organizer related genes (Lhx1, Cer1 and Foxa2) was specifically induced at day 3 of EBs, but not in monolayer culture. In epiblast model cells, the overexpression of Lhx1 induced gene expression of organizer and meso-endodermal markers, suggested that Lhx1 was related to epiblast differentiation.
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| Overall design |
Gene expression related epiblast development was measured at undifferentiated state and day 1-3 or 4 of differentiation. Two independent experiments were performed at each time using different people for each experiment.
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| Contributor(s) |
Ikeda N, Hisatome I, Shirayoshi Y |
| Citation missing |
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| |
| Submission date |
Jan 07, 2015 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Akira Hasegawa |
| E-mail(s) |
ahase@med.tottori-u.ac.jp
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| Organization name |
Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science,Tottori University
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| Department |
Department of Genetic Medicine and Regenerative Therapeutics
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| Lab |
Division of Regenerative Medicine and Therapeutics
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| Street address |
36-1, Nishimachi
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| City |
Yonago |
| State/province |
Tottori |
| ZIP/Postal code |
683-8503 |
| Country |
Japan |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA271729 |
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