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Series GSE64684 Query DataSets for GSE64684
Status Public on Oct 01, 2015
Title Extensive Nuclear Reprogramming Underlies Lineage Conversion into Functional Trophoblast Stem-like Cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The generation of induced pluripotent stem cells (iPSCs) and the direct conversion approach provide an invaluable resource of cells for disease modeling, drug screening, and patient-specific cell-based therapy. However, while iPSCs are stable and resemble ESCs in their transcriptome, methylome and function, the vast majority of the directly converted cells represent an incomplete reprogramming state as evident by their aberrant transcriptome and transgene dependency. This raises the question of whether complete and stable nuclear reprogramming can be achieved only in pluripotent cells. Here we demonstrate the generation of stable and fully functional induced trophoblast stem cells (iTSCs) by transient expression of Gata3, Tfap2c and Eomes. Similarly to iPSCs, iTSCs underwent a complete and stable reprogramming process as assessed by transcriptome and methylome analyses and functional assays such as the formation of hemorrhagic lesion and placenta contribution. Careful examination of the conversion process indicated that the cells did not go through a transient pluripotent state. These results suggest that complete nuclear reprograming can be attained in non-pluripotent cells.
Overall design Technical duplicates of 10 samples
Contributor(s) Benchetrit H, Buganim Y
Citation(s) 26412562
Submission date Jan 05, 2015
Last update date Dec 27, 2021
Contact name Yossi buganim
Organization name The Hebrew University of Jerusalem
Department Developmental Biology and Cancer Research
Lab Buganim
Street address Ein Karem 91120
City Jerusalem
ZIP/Postal code 91120
Country Israel
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (10)
GSM1576848 12F-iTSC#5
GSM1576849 12F-iTSC#4
GSM1576850 4F-iTSC#3
BioProject PRJNA271620
SRA SRP051710

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Supplementary file Size Download File type/resource
GSE64684_RAW.tar 16.9 Mb (http)(custom) TAR (of TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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