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Series GSE6465 Query DataSets for GSE6465
Status Public on Nov 01, 2008
Title Expression data of Hepatocellular Carcinoma
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Expression profiling of Xenografts of Hepatocellular Carcinoma
Keywords: Human Cancer
 
Overall design Briefly, primary HCCs obtained during liver resection were minced into fine fragments capable of passing through an 18-gauge needle, mixed in a 1:1 (v/v) ratio with Matrigel (Collaborative Research, Bedford, MA) and injected in the subcutaneous flanks of 8-week-old male severe combined immune deficient (SCID/SCID, The Jackson Laboratory, Harbor, ME) mice. Six to eight mice were injected for each primary tumor. Growth of established tumor xenografts was monitored twice weekly by vernier caliper measurement of tumor length (a) and width (b), and tumor volume was calculated as (a x b2) / 2. Serial passages of xenograft lines were obtained by dissecting tumors from sacrificed animals and reinjecting the dissociated tumor cells into successive generations of SCID mice as described above. Seven xenograft lines were studied in this report (2-1318, 5-1318(1), 5-1318(3), 2006, 26-1004, 26-1004(cirr), and 30-1004). Xenografts 2-1318 and 5-1318(1) were derived from Hep B-positive patients.
 
Contributor(s) Yu K, Tan P
Citation(s) 18490075
Submission date Dec 06, 2006
Last update date Mar 25, 2019
Contact name Kun Yu
E-mail(s) yukun@omniarray.com
Phone +65 64368390
Organization name National Cancer Centre
Department Cellular & Molecular Research
Street address
City Singapore
ZIP/Postal code 169610
Country Singapore
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (53)
GSM148594 Cell line Hep3B(a)
GSM148595 Cell line HepG2(b)
GSM148596 Cell line HK-Hep1(b)
Relations
BioProject PRJNA98697

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6465_RAW.tar 219.0 Mb (http)(custom) TAR (of CEL)

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