|Public on Mar 02, 2023
|Genome activity during the emergence of mouse embryonic totipotency (morpholino)
|Expression profiling by array
|Fertilization transforms sperm and egg into a totipotent embryo but the underlying mechanisms are unknown. We here report that gene expression initiates during the gamete-to-embryo transition in mouse embryos. Meiotic exit induced by sperm entry enhances a transcriptionally-permissive epigenetic landscape. Time-course analysis of single embryos revealed a succession of genome-wide transcription 'ripples' initiating within 2 hours. Disrupting key pluripotency transcription factor levels prior to sperm entry had little immediate effect, indicating that different mechanisms engender pluripotent and totipotent states. These findings suggest that a hierarchical gene expression program characterizes the emergence of totipotency during the gamete-to-embryo transition, with broad mechanistic implications for the reprogramming of cellular potency.
|Two-condition experiment in each Morpholino and cRNA mII injected mouse embryos, Morpholino control vs. Morpholino Oct4A and mcherry cRNA vs Oct4A-mcherry cRNA. Biological replicates: 8 control replicates, 12 Morpholino Oct4A replicates, 12 control cRNA replicates, 11 Oct4A-mcherry cRNA replicates.
|Jan 05, 2015
|Last update date
|Mar 03, 2023
|University of Regensburg
|Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
|13h post ICSI single zygote injected Morpholino control-replicate 01
|13h post ICSI single zygote injected Morpholino control-replicate-02
|13h post ICSI single zygote injected Morpholino control-replicate-03
|This SubSeries is part of SuperSeries:
|Genome activity during the emergence of mouse embryonic totipotency