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Series GSE64648 Query DataSets for GSE64648
Status Public on Mar 02, 2023
Title Genome activity during the emergence of mouse embryonic totipotency (morpholino)
Organism Mus musculus
Experiment type Expression profiling by array
Summary Fertilization transforms sperm and egg into a totipotent embryo but the underlying mechanisms are unknown. We here report that gene expression initiates during the gamete-to-embryo transition in mouse embryos. Meiotic exit induced by sperm entry enhances a transcriptionally-permissive epigenetic landscape. Time-course analysis of single embryos revealed a succession of genome-wide transcription 'ripples' initiating within 2 hours. Disrupting key pluripotency transcription factor levels prior to sperm entry had little immediate effect, indicating that different mechanisms engender pluripotent and totipotent states. These findings suggest that a hierarchical gene expression program characterizes the emergence of totipotency during the gamete-to-embryo transition, with broad mechanistic implications for the reprogramming of cellular potency.
 
Overall design Two-condition experiment in each Morpholino and cRNA mII injected mouse embryos, Morpholino control vs. Morpholino Oct4A and mcherry cRNA vs Oct4A-mcherry cRNA. Biological replicates: 8 control replicates, 12 Morpholino Oct4A replicates, 12 control cRNA replicates, 11 Oct4A-mcherry cRNA replicates.
 
Contributor(s) Perry AC
Citation(s) 36729835
Submission date Jan 05, 2015
Last update date Mar 03, 2023
Contact name Xin Lu
E-mail(s) luxgmpg@gmail.com
Organization name University of Regensburg
Street address Universit├Ątsstra├če 31
City Regensburg
ZIP/Postal code 93053
Country Germany
 
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (31)
GSM1576181 13h post ICSI single zygote injected Morpholino control-replicate 01
GSM1576182 13h post ICSI single zygote injected Morpholino control-replicate-02
GSM1576183 13h post ICSI single zygote injected Morpholino control-replicate-03
This SubSeries is part of SuperSeries:
GSE64650 Genome activity during the emergence of mouse embryonic totipotency
Relations
BioProject PRJNA271564

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE64648_RAW.tar 143.4 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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