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Series GSE64622 Query DataSets for GSE64622
Status Public on Jul 31, 2015
Title Conversion of MyoD to a neurogenic factor: binding site specificity determines lineage [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary MyoD and NeuroD2 are master regulators of myogenesis and neurogenesis and bind to a "shared" E-box sequence (CAGCTG) and a "private" sequence (CAGGTG or CAGATG, respectively). To determine whether private-site recognition is sufficient to confer lineage-specification, we generated a MyoD-mutant with the DNA binding specificity of NeuroD2. Our results demonstrate that redirecting MyoD binding from MyoD-private sites to NeuroD2-private sites, despite preserved binding to the MyoD/NeuroD2-shared sites, is sufficient to change MyoD from a master regulator of myogenesis to a master regulator of neurogenesis.
Overall design RNA-seq profiling of mouse P19 cells transfected with MyoD, NeuroD2 and chimera mutants. The chimeric mutants are MyoD with the bHLH domain replaced with the NeuroD2 bHLH domain.
Contributor(s) Fong A, Yao Z, Zhong JW, Tapscott S
Citation(s) 25801030
Submission date Jan 02, 2015
Last update date May 15, 2019
Contact name Stephen Tapscott
Organization name Fred Hutch Cancer Research Center
Department Human Biology
Lab Tapscott
Street address 1100 Fairview N. Ave
City Seattle
State/province WASHINGTON
ZIP/Postal code 98103
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (8)
GSM1575795 GFP
GSM1575796 MD
GSM1575797 MDND2
This SubSeries is part of SuperSeries:
GSE64627 Conversion of MyoD to a neurogenic factor: binding site specificity determines lineage
BioProject PRJNA272314
SRA SRP051986

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Supplementary file Size Download File type/resource
GSE64622_gene.counts.txt.gz 404.1 Kb (ftp)(http) TXT
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