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Series GSE64582 Query DataSets for GSE64582
Status Public on Dec 31, 2014
Title SNP data from human fibroblasts and induced Pluripotent Stem cells from amyotrophic lateral sclerosis patients
Organism Homo sapiens
Experiment type SNP genotyping by SNP array
Summary We have generated human induced Pluripotent Stem cells (hiPSc) from amyotrophic lateral sclerosis (ALS, motor neuron disease) patients, using Sendai virus-mediated delivery of reprogramming factors. hiPSc lines have been screened using SNP array to assess chromosomal stability (alongside the fibroblast lines from which they derived), and validation of the pluripotency of the hiPSc lines is provided by Pluritest assessment of transcriptome datasets, prior to differentiation to motor neuron cultures and downstream functional assays.
Mutihac R., Scaber J., Lalic T., Ababneh N., Vowles, J., Fletcher-Jones A., Douglas A.G.L., Browne C., Nakanishi M., Turner M., Wade-Martins R., Cowley S.A. and Talbot K. Altered ER calcium homeostasis and stress granule formation in iPSC-derived motor neurons from ALS/FTD patients with C9orf72 expansions. Submitted
Overall design human iPSc lines were derived from human dermal fibroblasts from 3 ALS patients with C9orf72 mutations (2 lines per patient) and control donors. SNP datasets for control fibroblasts HDF OX1 and NHDF, and SNP and gene expression datasets from control lines iPS OX1-19 and iPS NHDF-1, have been published previously: Van Wilgenburg B., Browne C., Vowles J. & Cowley S. A. Efficient, long term production of monocyte-derived macrophages from human pluripotent stem cells under partly-defined and fully-defined conditions. PLoS One 8, e71098 (2013). GEO Superseries GSE45472 ; Physiological characterisation of human iPS-derived dopaminergic neurons. Hartfield EM, Yamasaki-Mann M, Ribeiro Fernandes HJ, Vowles J, James WS, Cowley SA, Wade-Martins R.PLoS One. 2014 9(2):e87388 GEO superseries GSE43904. SNP dataset for control fibroblasts HDF AH017 is in press, and is available in GEO Superseries GSE69302.
Contributor(s) Mutihac R, Scaber J, Lalic T, Ababneh N, Vowles J, Fletcher-Jones A, Douglas AG, Browne C, Nakanishi M, Turner M, Wade-Martins R, Cowley SA, Talbot K
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Submission date Dec 30, 2014
Last update date Dec 18, 2017
Contact name Sally A Cowley
Phone +44 (0) 1865 275600
Organization name University of Oxford
Department Sir William Dunn School of Pathology
Lab James Martin Stem Cell Facility
Street address South Parks Road
City Oxford
ZIP/Postal code OX1 3RE
Country United Kingdom
Platforms (2)
GPL13829 Illumina HumanCytoSNP-12 v2.1 BeadChip
GPL19699 HumanOmniExpress-24 v1.0 BeadChip
Samples (13)
GSM1574698 HDF C9_T2
GSM1574699 iPS C9_T2_6
GSM1574700 iPS C9_T2_7
This SubSeries is part of SuperSeries:
GSE64584 Functional and pathological features of ALS in iPSC-derived motor neurons from patients with C9orf72 expansions
BioProject PRJNA271355

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE64582_RAW.tar 72.2 Mb (http)(custom) TAR
GSE64582_signal_intensities_GPL13829.txt.gz 32.1 Mb (ftp)(http) TXT
GSE64582_signal_intensities_GPL19699.txt.gz 47.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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