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Series GSE64553 Query DataSets for GSE64553
Status Public on Aug 26, 2015
Title RNA-seq of human fibroblasts during normal aging and during aging with rotenone perturbation
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Human fibroblasts at different population doublings were treated with low amounts of rotenone (mild stress) and compared to untreated fibroblasts. Two different cell lines were used (MRC-5, HFF). Illumina sequencing (HiSeq2000) was applied to generate 50bp single-end reads.

Jena Centre for Systems Biology of Ageing - JenAge (
Overall design 60 samples: 3 biological replicates for each group: MRC-5 cells at 4 different population doublings (PD) with and without rotenone; HFF cells at 6 different population doublings with and without rotenone
Contributor(s) Marthandan S, Klement K, Priebe S, Groth M, Platzer M, Hemmerich P, Diekmann S
Citation(s) 26380578
Submission date Dec 29, 2014
Last update date May 15, 2019
Contact name JenAge Project
Organization name Leibniz Institute for Age Research - Fritz Lipmann Institute
Street address Beutenbergstr. 11
City Jena
ZIP/Postal code D-07745
Country Germany
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (60)
GSM1574089 MRC5_PD38wo_1
GSM1574090 MRC5_PD38wo_2
GSM1574091 MRC5_PD38wo_3
BioProject PRJNA271291
SRA SRP051599

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE64553_counts_rpkm_exvivo_rotenone_data.txt.gz 3.5 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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