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Series GSE64550 Query DataSets for GSE64550
Status Public on Dec 30, 2014
Title shRNA knockdown of YAP1 in HCC364 cells, various drug conditions
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Through a genetic screen in BRAF mutant tumor cells, we show that the Hippo pathway effector YAP acts as a parallel survival input to promote resistance to RAF-MEK inhibitor therapy. Our data uncover YAP as a novel mechanism of resistance to RAF-MEK targeted therapy. The findings unveil the synthetic lethality of YAP and RAF-MEK co-suppression as a promising strategy to enhance response and patient survival.
Overall design RNAseq analysis of HCC364 (lung adenocarcinoma) cells in the context of drug treatment with PLX4720 (vemurafenib, a BRAF inhibitor) or Trametinib (a MEK inhibitor) alongside shRNA knockdown of the gene YAP1
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Submission date Dec 29, 2014
Last update date May 15, 2019
Contact name Saurabh Asthana
Organization name UCSF
Department Helen Diller Family Comprehensive Cancer Center
Lab Olshen
Street address 1450 Third Street, Room HD276
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (9)
GSM1574056 hcc364_dmso
GSM1574057 hcc364_plx
GSM1574058 hcc364_tra
BioProject PRJNA271287
SRA SRP051595

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE64550_RAW.tar 13.3 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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