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Series GSE64513 Query DataSets for GSE64513
Status Public on Dec 24, 2015
Title FOXD3 is a novel tumor suppressor in lung cancer
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The transcription factor forkhead box D3 (FoxD3) is a transcriptional factor which belongs to forkhead box (Fox) transcription factor family. The functions of FOXD3 in embryogenesis and in the development of neural crest cells have been clearly defined. Its tumor suppressor function hasbeen found in many types of cancer in recent years. However, the study about its roles in lung cancerdevelopment is still lacking. Our study found that deficiency of FoxD3 in lung cancer enhanced cell growth and cell invasion. RNA-sequence analysis demonstrated that loss of FoxD3 mainly affected cell cycle progression related gene expression. Knockdown of FoxD3 led to G2-M cell accumulation with up-regulation of DNA replication licensing factor MCM5 and MCM4 as well as cell cycle regulator polo-like kinase-1 (PLK1) and CDC6. Over-expression of those genesin lung cancer was associated with poor clinical outcomes of lung cancer patients. We also identified high mobility group box-1(HMGB1), Hras and Ephrin B1 gene expression increase after FoxD3 silencing which may participate in enhanced lung cancer cell invasion. Our study identifiedtumor suppressor function of FoxD3 in lung cancer andwe did the first comprehensive analysis of the genes regulated by FoxD3 for cell proliferation and invasion in lung cancer. The identified genes regulated by FoxD3 through our analysis will provide valuable information to uncover the mechanism of FoxD3 tumor suppressor function.
Overall design Three control and three FOXD3 knockdown A549 cell lines were subjected to RNA sequencing.
Contributor(s) Xiao J, Xu W
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Submission date Dec 24, 2014
Last update date May 15, 2019
Contact name Jianru Xiao
Organization name Changzheng Hospital, Second Military Medical University
Department Department of Orthopedics Oncology
Street address 415 Fengyang Road
City Shanghai
ZIP/Postal code 200003
Country China
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (6)
GSM1573115 WGC023775R
GSM1573116 WGC023776R
GSM1573117 WGC023777R
BioProject PRJNA271162
SRA SRP051544

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE64513_FOXD3_vs_Control_gene_exp_diff_anno.txt.gz 1.4 Mb (ftp)(http) TXT
GSE64513_FOXD3_vs_Control_gene_exp_diff_sig_anno.txt.gz 143.7 Kb (ftp)(http) TXT
GSE64513_RAW.tar 10.8 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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