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Series GSE64430 Query DataSets for GSE64430
Status Public on Feb 27, 2015
Title Effect of cajanin stilbene acid and its synthetic derivatives on MCF-7 breast cancer cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Cajanin stilbene acid (CSA) was previously shown to induce cytotoxicity against cancer cells. We have employed microarray gene expression profiling to identify deregulated genes in MCF-7 breast cancer cells upon treatment with CSA and its synthetic derivatives (CSA6, CSA9, CSA19). TGM2 gene encoding transglutaminase 2 was commonly deregulated after treatment with all four CSA compounds. All compounds revealed strong effects on cell cycle progression and DNA damage response. Promoter motif analysis of the deregulated genes further supported the microarray results emphasizing the relevance of transcription factors regulating cell cycle and proliferation, MYC being among the most pronounced ones. Interestingly, cellular movement was among the top five affected cellular functions after treatment with the four derivatives.
Overall design Gene expression profiling study with Whole Human Genome RNA chips (8×60K Agilent) was performed in MCF-7 breast cancer cells upon treatment with CSA and its synthetic derivatives (CSA6, CSA9, CSA19). Twelve chips were used; control (x2), CSA (x2), control (x2), CSA6 (x2), CSA9 (x2), CSA19 (x2). MCF-7 cells were treated with the compounds (IC50 concentrations) for 72 hours or left untreated (DMSO control). Two independent experiments were performed for each treatment.
Contributor(s) Kadioglu O, Fu Y, Wiench B, Zu Y, Efferth T
Citation(s) 25716159
Submission date Dec 22, 2014
Last update date Nov 27, 2018
Contact name Onat Kadioglu
Organization name Johannes Gutenberg University
Department Institute of Pharmacy and Biochemistry
Street address Staudinger Weg 5
City Mainz
ZIP/Postal code 55099
Country Germany
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (12)
GSM1571194 DMSO control replicate 1
GSM1571195 DMSO control replicate 2
GSM1571196 CSA treatment replicate 1
BioProject PRJNA271005

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE64430_RAW.tar 42.2 Mb (http)(custom) TAR (of TXT)
GSE64430_processed_data.txt.gz 895.0 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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