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Series GSE64243 Query DataSets for GSE64243
Status Public on Jan 31, 2016
Title A ChIP-seq spike-in method enables detection of global histone modification changes across the genome
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary This study outlines a method that dramatically alters the interpretation of ChIP-seq data and will improve the quantitative comparison of histone modification maps across biological contexts or across various conditions within a given biological context.
Overall design We introduced a small fraction of Drosophila chromatin into human ChIP samples and added a Drosophila-specific antibody as a means to consistently precipitate Drosophila chromatin as a minor fraction of the total ChIP DNA. The Drosophila ChIP-seq tags are used to normalize the human ChIP-seq data from DMSO and EZH2 inhibitor-treated samples. Employing this strategy, a substantial reduction in H3K27me3 levels is observed across the genome upon EZH2 inhibitor treatment.
Contributor(s) Egan B, Craske M, Labhart P, Yuan C, Guler GD, Arnott D, Maille T, Busby J, Henry C, Kelly TK, Tindell C, Jhunjhunwala S, Zhao F, Hatton C, Bryant BM, Classon M, Trojer P
Citation(s) 27875550
Submission date Dec 16, 2014
Last update date May 15, 2019
Contact name Suchit Jhunjhunwala
Organization name Genentech
Street address 1 DNA Way, MS-444A
City South San Francisco
State/province CA
ZIP/Postal code 94080
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (8)
GSM1566893 PC9_control_H3K27me3
GSM1566894 PC9_EZH2inh_H3K27me3
GSM1566895 PC9_control_H3K4me3
BioProject PRJNA270645
SRA SRP051259

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Supplementary file Size Download File type/resource
GSE64243_RAW.tar 4.6 Mb (http)(custom) TAR (of BED)
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Raw data are available in SRA
Processed data provided as supplementary file

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