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Series GSE64113 Query DataSets for GSE64113
Status Public on Aug 03, 2015
Title Comparison of poly(A) and capture RNA-seq: controlled degradation in vitro
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We compare the performance of two library preparation protocols (poly(A) and exome capture) in in vitro degraded RNA samples
Overall design VcaP cell were grown, and treated with MDV3100 (enzalutamide) or DHT (dihydrotestosterone), intact RNA was isolated and samples were prepared in technical triplicates using two library preparation protocol. Also cells were subject to in vitro degradation through incubation of the whole cell lysate in 37C for increasing amounts of time. Following incbation paired capture and poly(A) libraries were prepared.
Contributor(s) Cieslik MP, Chinnaiyan AM
Citation(s) 26253700
Submission date Dec 12, 2014
Last update date May 15, 2019
Contact name Marcin Piotr Cieslik
Organization name University of Michigan
Department Pathology
Street address 500 S State St
City Ann Arbor
State/province Michigan
ZIP/Postal code 48104
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (40)
GSM1564635 SI_8064-8-3.3-mdv-capture
GSM1564636 SI_8063-8-3-mdv-capture
GSM1564637 SI_8062-8-3.6-mdv-capture
BioProject PRJNA270181
SRA SRP051102

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Supplementary file Size Download File type/resource
GSE64113_RAW.tar 73.5 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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