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Series GSE64092 Query DataSets for GSE64092
Status Public on Nov 23, 2016
Title RNA-seq profiling of Hoxa2/Hoxb2 mutants versus wild type in Math1 positive cells from the Cochlear Nucleus
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Differential gene expression was analyzed for FACS sorted Math1::Cre; ROSA-tdTomato from hand dissected cochlear nuclei of wild type and Hoxa2/Hoxb2 mutant mice
Overall design In order to investigate the role of Hoxa2 and Hoxb2 transcription factors in a subset of cells of the cochlear nucleus, we generated double conditional knock-out by crossing the deleter line Math1::Cre crossed with Rosa tdTomato; Hoxa2fl/fl; Hoxb2fl/fl and Rosa tdTomato wild type background. FACS sorted cells from hand dissected cochlear nuclei were than processed and RNA-seq performed (see extract protocol and library construction protocol).
Contributor(s) Loche A, Karmakar K, Rijli F, Thiry S
Citation(s) 28052248
Submission date Dec 11, 2014
Last update date May 15, 2019
Contact name Tim Roloff
Organization name FMI
Department Functional Genomics
Street address Maulbeerstrasse 66
City Basel
ZIP/Postal code 4058
Country Switzerland
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (8)
GSM1564180 Math1::Cre; Rosa tdTomato rep 1
GSM1564181 Math1::Cre; Rosa tdTomato rep 2
GSM1564182 Math1::Cre; Rosa tdTomato rep 3
BioProject PRJNA270100
SRA SRP051077

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE64092_Raw_Read_Counts_mm10_Ensembl_genes.txt.gz 550.4 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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