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Series GSE64008 Query DataSets for GSE64008
Status Public on Jun 19, 2015
Title Nucleoporin Nup153 Regulates Stem Cell Pluripotency through Gene Silencing
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Nucleoporins (Nups) are a family of proteins best known as the constituent building blocks of nuclear pore complexes (NPCs), the transport channels that mediate nuclear transport. Recent evidence suggest that several Nups have additional roles in controlling the activation and silencing of developmental genes, however, the mechanistic details of these functions remain poorly understood. Here, we show that depletion of Nup153 in mouse embryonic stem cells (mESCs) causes the de-repression of developmental genes and induction of early differentiation. This loss of pluripotency is not associated with defects in global nucleo-cytoplasmic transport activity. Instead, Nup153 binds to the transcriptional start site (TSS) of developmental genes and mediates the recruitment of the polycomb repressive complex 1 (PRC1) to its target loci. Our results reveal a nuclear transport-independent role of Nup153 in maintaining stem cell pluripotency and introduce a role of NPC proteins in mammalian epigenetic gene silencing.
Overall design RNA-seq, ChIP-Seq, and DamID-Seq for Nup153, Oct4, and key chromatin regulators in mouse ES cells and neural progenitors
Contributor(s) Jacinto FV, Benner C, Hetzer MW
Citation(s) 26080816
Submission date Dec 09, 2014
Last update date May 15, 2019
Contact name Christopher Benner
Organization name University of California, San Diego (UCSD)
Department Medicine
Street address 9500 Gilman Dr. MC 0640
City La Jolla
State/province California
ZIP/Postal code 92093-0640
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (19)
GSM1562325 Nup153-DamID-r1
GSM1562326 Nup153-DamID-r2
GSM1562327 Oct4-DamID-r1
BioProject PRJNA269747
SRA SRP050985

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Supplementary file Size Download File type/resource
GSE64008_Cbx7.shCtrl.bed.gz 164.9 Kb (ftp)(http) BED
GSE64008_Nup153.DamID.bed.gz 47.9 Kb (ftp)(http) BED
GSE64008_Oct4.DamID.bed.gz 48.9 Kb (ftp)(http) BED
GSE64008_Oct4.ctrl.bed.gz 19.2 Kb (ftp)(http) BED
GSE64008_Oct4.shNup153.bed.gz 20.9 Kb (ftp)(http) BED
GSE64008_Ring1B.NeuP.bed.gz 193.2 Kb (ftp)(http) BED
GSE64008_Ring1B.shCtrl.bed.gz 151.2 Kb (ftp)(http) BED
GSE64008_Ring1B.shNup153-2.bed.gz 208.0 Kb (ftp)(http) BED
GSE64008_Ring1B.shNup153-4.bed.gz 155.1 Kb (ftp)(http) BED
GSE64008_fpkm.txt.gz 1.3 Mb (ftp)(http) TXT
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Processed data are available on Series record

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