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Status |
Public on May 20, 2015 |
Title |
The carbonic anhydrase inhibitor ethoxzolamide inhibits the Mycobacterium tuberculosis PhoPR regulon and Esx-1 secretion and attenuates virulence |
Organism |
Mycobacterium tuberculosis |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
The purpose of this study was to examine how ethoxzolamide modulates gene M. tuberculosis gene expression at acidic pH. We observed that ethoxzolamide downregulates genes of the PhoPR regulon.
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Overall design |
Mtb strain CDC1551 was grown at 37C in T-25 vented, standing tissue culture flasks in 8 ml of buffered 7H9 medium seeded an initial OD of 0.2. Three conditions were examined with two biological replicates: 1) DMSO treated, pH 7.0, 2) DMSO treated, pH 5.7, 3) 40 µM ethoxzolamide treated, pH 5.7. The CDC1551(phoP::Tn) mutant was grown in a similar manner and treated with DMSO at pH 7.0 and 5.7. Following six days incubation, total bacterial RNA was extracted and analyzed as previously described.
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Contributor(s) |
Johnson BK, Colvin CJ, Abramovitch RB |
Citation(s) |
25987613 |
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Submission date |
Dec 05, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Robert B. Abramovitch |
Organization name |
Michigan State University
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Department |
Microbiology and Molecular Genetics
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Street address |
567 Wilson Road
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City |
East Lansing |
State/province |
MI |
ZIP/Postal code |
48824 |
Country |
USA |
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Platforms (1) |
GPL17879 |
Illumina HiSeq 2500 (Mycobacterium tuberculosis) |
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Samples (8)
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GSM1560344 |
WT, DMSO, pH 5.7, RepB |
GSM1560345 |
WT, Ethoxzolamide, pH 5.7, Rep A |
GSM1560346 |
WT, Ethoxzolmaide, pH 5.7, Rep B |
GSM1560347 |
phoP::Tn, DMSO, pH 5.7, Rep A |
GSM1560348 |
phoP::Tn, DMSO, pH 5.7, Rep B |
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Relations |
BioProject |
PRJNA269492 |
SRA |
SRP050863 |
Supplementary file |
Size |
Download |
File type/resource |
GSE63917_SupplementalTable1A_DMSO_pH5.7_v_pH7_induction.txt.gz |
8.2 Kb |
(ftp)(http) |
TXT |
GSE63917_SupplementalTable1B_DMSO_pH5.7_v_pH7_repression.txt.gz |
11.0 Kb |
(ftp)(http) |
TXT |
GSE63917_SupplementalTable2A_ETZ_v_DMSO_pH5.7_induction.txt.gz |
782 b |
(ftp)(http) |
TXT |
GSE63917_SupplementalTable2B_ETZ_v_DMSO_pH5.7_repression.txt.gz |
3.2 Kb |
(ftp)(http) |
TXT |
GSE63917_SupplementalTable3A_PhoPTn_v_DMSO_pH5.7_induction.txt.gz |
4.8 Kb |
(ftp)(http) |
TXT |
GSE63917_SupplementalTable3B_PhoPTn_v_DMSO_pH5.7_repression.txt.gz |
11.8 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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