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Series GSE63872 Query DataSets for GSE63872
Status Public on Jan 16, 2015
Title Human Promoters Are Intrinsically Directional
Organism Homo sapiens
Experiment type Other
Summary Divergent transcription, in which reverse-oriented transcripts occur upstream of eukaryotic promoters in regions devoid of annotated genes, has been suggested to be a general property of active promoters. Here we show that the human basal RNA polymerase II transcriptional machinery and core promoter are inherently unidirectional, and that reverse-oriented transcripts originate from their own cognate reverse-directed core promoters. In vitro transcription analysis and mapping of nascent transcripts in cells revealed that core promoters are unidirectional and that sequences at reverse start sites are similar to those of their forward counterparts. The use of DNase I accessibility to define proximal promoter borders revealed that about half of promoters are unidirectional and that these unidirectional promoters are depleted at their upstream edges of reverse core promoter sequences and their associated chromatin features. Divergent transcription is thus not an inherent property of the transcription process, but rather the consequence of the presence of both forward- and reverse-directed core promoters.
Overall design Using 5'-GRO-seq and GRO-seq to determine mechanisms of divergent transcription initiation
Contributor(s) Duttke SH, Lacadie SA, Ibrahim MM, Glass CK, Corcoran DL, Benner C, Heinz S, Kadonaga JT, Ohler U
Citation(s) 25639469
Submission date Dec 04, 2014
Last update date May 15, 2019
Contact name Scott Allen Lacadie
Organization name Max Delbrück Center for Molecular Medicine
Department Berlin Institute for Medical Systems Biology
Lab Ohler
Street address Robert-Rössle-Str. 10
City Berlin-Buch
ZIP/Postal code 13092
Country Germany
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (2)
GSM1558745 HelaS3_5'-GRO-seq
GSM1558746 HelaS3_GRO-seq
BioProject PRJNA269295
SRA SRP050563

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Supplementary file Size Download File type/resource
GSE63872_RAW.tar 101.5 Mb (http)(custom) TAR (of BED, BEDGRAPH)
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Raw data are available in SRA
Processed data provided as supplementary file

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