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Series GSE63861 Query DataSets for GSE63861
Status Public on Nov 04, 2015
Title High Mobility Group protein mediated transcription requires DNA damage marker γ-H2AX
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The eukaryotic genome is organized into chromatin, which constitutes the physiological template for DNA-dependent processes including replication, recombination, repair and transcription. Chromatin mediated transcription regulation involves histone modifications, chromatin remodeling and DNA methylation. However, the precise biological function of non-histone chromatin-associated proteins is still unclear. The high mobility group proteins are the most abundant non-histone chromatin-associated proteins. Here we combined proteomic, ChIP-seq and transcriptome data to decipher the mechanism of transcriptional regulation mediated by the high mobility group AT-hook protein 2 (HMGA2). We showed that HMGA2-induced transcription requires H2AX phosphorylation at S139 (H2AXS139ph; γ-H2AX), mediated by the kinase ataxia telangiectasia mutated (ATM). Furthermore, we demonstrated the relevance of this mechanism within the biological context of TGFB1-signaling. Our results link H2AXS139ph, a marker for DNA damage, to transcription, which is a new function for this histone modification. The interplay between HMGA2, ATM and H2AX is a novel mechanism of transcription initiation.
Overall design Chip-seq data of HMGA2, H2AXS139ph and ATM obtained from Mouse embryonic Fibroblast cells in wt and Ko of Hmga2
Contributor(s) Barreto G, Singh I, Cordero J, Ozturk N
Citation(s) 26045162
Submission date Dec 04, 2014
Last update date May 15, 2019
Contact name Guillermo Barreto
Organization name CNRS Director of Research
Department Campus Biologie-Santé. Faculté de Médecine
Lab Laboratoire IMoPA. UMR 7365 CNRS
Street address 9, Avenue de la Forêt de Haye ‐ BP 20199
City Nancy Cedex
ZIP/Postal code 54505
Country France
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (8)
GSM1558587 HMGA2_Chip_seq_in_MEF_Hmga2_wt
GSM1558588 H2AXS139ph_Chip_seq_in_MEF_Hmga2_wt
GSM1558589 ATM_Chip_seq_in_MEF_Hmga2_wt
BioProject PRJNA269287
SRA SRP050556

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE63861_01_contigs_ATM_in_Hmga2_MEF_wt_vs_ATM_in_Hmga2_MEF_ko_in_promoter_wt.txt.gz 357 b (ftp)(http) TXT
GSE63861_01_contigs_H2AXS139ph_in_Hmga2_MEF_wt_vs_H2AXS139ph_in_Hmga2_MEF_Ko.txt.gz 373 b (ftp)(http) TXT
GSE63861_ATM_in_Hmga2_MEF_wt_peaks.bed.gz 1.5 Mb (ftp)(http) BED
GSE63861_ATM_promoter_in_Hmga2_MEF_wt.bed.gz 7.8 Kb (ftp)(http) BED
GSE63861_H2AXS139ph_and_ATM_in_Hmga2_MEF_wt_compared_HMGA2_in_MEF_Hmga2_wt_peaks.txt.gz 170.8 Kb (ftp)(http) TXT
GSE63861_H2AXS139ph_in_Hmga2_MEF_wt_peaks.bed.gz 1.5 Mb (ftp)(http) BED
GSE63861_H2AXS139ph_promoter_in_Hmga2_MEF_wt.bed.gz 19.3 Kb (ftp)(http) BED
GSE63861_HMGA2_in_MEF_Hmga2_wt_peaks.bed.gz 1.9 Mb (ftp)(http) BED
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