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Series GSE63800 Query DataSets for GSE63800
Status Public on Dec 05, 2014
Title The Sm protein methyltransferase PRMT5, is not required for primordial germ cell specification in mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary PRMT5 is a type II protein arginine methyltransferase with roles in stem cell biology, reprogramming, cancer and neurogenesis. During embryogenesis in the mouse it was hypothesized that PRMT5 functions with the master germline determinant BLIMP1 to promote primordial germ cell (PGC) specification. Using a Blimp1-Cre germline conditional knockout, we discovered that Prmt5 has no major role in murine germline specification, or the first global epigenetic reprogramming event involving depletion of cytosine methylation from DNA and histone H3 lysine 9 dimethylation from chromatin. Instead, we discovered that PRMT5 functions at the conclusion of PGC reprogramming I to promote proliferation, survival and expression of the gonadal germline program as marked by MVH. We show that PRMT5 regulates gene expression by promoting methylation of the Sm spliceosomal proteins, and significantly altering the spliced repertoire of RNAs in mammalian embryonic cells and primordial cells.
Overall design Examination of transcriptional profile of iPHet (Control) vs. iPKO (Prmt5 knock out) 2i Embryonic Stem Cells
Contributor(s) Clark AT, Li Z, Yu J
Citation(s) 25519955
BioProject PRJNA245758
Submission date Dec 03, 2014
Last update date May 15, 2019
Contact name Juehua Yu
Organization name UCLA
Street address 615 Charles E. Young South BSRB 457
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (8)
GSM1557609 iPHet 1
GSM1557610 iPHet 2
GSM1557611 iPHet 3
SRA SRP041516

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE63800_RAW.tar 8.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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