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Series GSE63743 Query DataSets for GSE63743
Status Public on Dec 13, 2014
Title Genome-Wide Mapping of 5-mC and 5-hmC in severely Preeclamptic Placentas.
Organism Homo sapiens
Experiment type Methylation profiling by high throughput sequencing
Summary Preeclampsia (PE) is a major contributor of maternal mortality with uncertain etiology. Recent studies suggested that epigenetic modifications, including DNA methylation, play a vital role in the development of PE. In this study, we have mapped genome-wide distribution of 5-methylcytosin (5-mC) and 5-hydroxymethylcytosine (5-hmC) using MeDIP and (h)MeDIP in the placentas from severely preeclamptic patients and normal controls. A total 194485 pooled 5-mC peaks and 138133 pooled 5-hmC peaks were identified, of which 714 5-mC peaks and 119 5-hmC peaks showed significant difference between patients and controls (>2-fold, p<0.05).To our knowledge, this is the first report of DHMRs (Differentially Hydroxy-Methylated Regions) in preeclamptic placenta. We not only confirmed the aberrant DNA methylated regions in the process of preeclampsia reported previously, but also identified unreported regions. A total of 4 selected DMRs (Differentially Methylated Regions) were also confirmed by MassARRAY EppiTYPER. Of these, PTPRN2, which had low level of 5-mC and high level of 5-hmC at gene body, was further verified to have lower methylation level at promoter regions in case group compared with controls. In conclusion, our study provided genome-wide distribution of 5-mC and 5-hmC in severe PE and normal controls, which have further clinical value for the identification of diagnostic and therapeutic markers for severe PE.
Overall design Examination of 5-mC and 5-hmC pattern in 4 control cases' tissue and 4 severely Preeclamptic Placentas cases' tissue.
Contributor(s) Xiaotian L, Fei L, Lisha Z, Ruitu L
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Submission date Dec 01, 2014
Last update date May 15, 2019
Contact name Li Xiaotian
Phone 18521304014
Organization name Obstetrics and Gynecology Hospital of Fudan University
Department Obstetrics Department
Lab Obstetrics Department
Street address Fangxie Road 419 in Huangpu District
City Shanghai
State/province Shanghai
ZIP/Postal code 200032
Country China
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (24)
GSM1556599 Sample_7_hMeDIP
GSM1556600 Sample_14_hMeDIP
GSM1556601 Sample_15_hMeDIP
BioProject PRJNA268963
SRA SRP050385

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SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE63743_RAW.tar 1.2 Gb (http)(custom) TAR (of BEDGRAPH)
GSE63743_Sample_MeDIP_pool_peaks.bed.gz 2.0 Mb (ftp)(http) BED
GSE63743_Sample_hMeDIP_pool_peaks.bed.gz 1.5 Mb (ftp)(http) BED
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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