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Series GSE63688 Query DataSets for GSE63688
Status Public on Mar 20, 2017
Title Mapping karyotypic heterogeneity in chromosomal instable murine T-cell acute lymphoblastic lymphomas using single-cell sequencing
Organism Mus musculus
Experiment type Other
Summary Chromosome instability (CIN) leads to aneuploidy and copy number variations (CNVs). Even though both are hallmarks of cancer cells, aneuploidy inhibits proliferation of untransformed cells, suggesting that cancer cells have adapted to cope with CIN. The spindle assembly checkpoint (SAC) prevents CIN by monitoring chromosome attachment and sister chromatid tension in mitosis. By conditionally inactivating Mad2, an essential SAC gene, we find that SAC inactivation in T-cells or hepatocytes is remarkably well tolerated and becomes tumorigenic when placed in a p53null or p53+/- predisposed background. The resulting T-ALLs and HCCs are highly aneuploid, exhibit clonal copy number changes that are tumor specific despite ongoing CIN, indicating that CIN is a powerful driver of tumor evolution.
Overall design Whole genome single cell sequencing was performed on individual T-cells from untransformed thymus or Mad2f/f p53f/f Lck-Cre+T-ALLs. Two individual tumours were sequenced, as well as an untransformed thymus control.
Contributor(s) Foijer F, Spierings D, Lansdorp P
Citation(s) 28318489
Submission date Nov 26, 2014
Last update date Mar 11, 2020
Contact name Floris Foijer
Organization name University Medical Center Groningen
Department European Institute for the Biology of Ageing
Lab Foijer lab
Street address Antonius Deusinglaan 1
City Groningen
ZIP/Postal code 9713AV
Country Netherlands
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (144)
GSM1555303 Mad2 f/f; p53 f/f; Lck-Cre+ T-ALL #1 10 Cell control
GSM1555304 Mad2 f/f; p53 f/f; Lck-Cre+ T-ALL #1 Cell 1
GSM1555305 Mad2 f/f; p53 f/f; Lck-Cre+ T-ALL #1 Cell 2
This SubSeries is part of SuperSeries:
GSE63689 Copy number changes (average and single cell) and matching transcriptomes of HCCs and T-ALLs isolated from Mad2 p53 conditional double knockout mice
BioProject PRJNA268693
SRA SRP050310

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Supplementary file Size Download File type/resource
GSE63688_processed_Illumina_Next_Gen_Data.xlsx 21.2 Kb (ftp)(http) XLSX
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Processed data are available on Series record

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