NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE63534 Query DataSets for GSE63534
Status Public on Jul 26, 2016
Title Transcriptome profiling of TALEN-mediated LATS2 knockout HeLa-S3 cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Chromatin modifying activities for construction of appropriate epigenetic landscapes by polycomb repressive complex 2 (PRC2) play an essential role in development and tumorigenesis. However, the spatiotemporal mechanisms by which PRC2 achieves diverse epigenomes for specific tissue or cellular contexts remain poorly understood. Here, we discovered that LATS2 knockout causes dysregulation of PRC2 and subsequent transcriptome changes for differentiation in both mouse and human cells. LATS2 depletion dependent dysregulation of PRC2 also effects H3K4me3 and forms negative feedback loop for maintenance of PRC2. Further analyses reveal that LATS2 on chromatin binds to EZH2 and LATS2 has ability to phosphorylate PRC2 in vitro. These LATS2 dependent H3K27me3 targets are highly induced during neurogenesis, and statistical analysis of glioblastoma multiforme reveals that LATS2-high cases show more dedifferentiated transcriptome and poor prognosis with silencing of H3K27me3 targets. These observations suggest that LATS2-mediated epigenome coordination is pivotal for development and disease, including cancer.
 
Overall design Three samples, two cell lines (WT_rep1, WT_rep2, and LATS2 KO) were analyzed by Agilent SurePrint G3 Human Gene Expression 8x60K v1 one color experiments.
 
Contributor(s) Okuzaki D, Torigata K
Citation(s) 27434182
Submission date Nov 21, 2014
Last update date Nov 27, 2018
Contact name Daisuke Okuzaki
E-mail(s) dokuzaki@biken.osaka-u.ac.jp
Phone +81-6-6879-4935
Organization name Osaka univ.
Department Immunology Frontier Research Center
Lab Human Immunology (Single Cell Genomics)
Street address Yamadaoka 3-1
City Suita
State/province Osaka
ZIP/Postal code 565-0871
Country Japan
 
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (3)
GSM1551883 HeLa-S3_parental_WT_rep1
GSM1551884 HeLa-S3_parental_WT_rep2
GSM1551885 HeLa-S3_LATS2_KO
This SubSeries is part of SuperSeries:
GSE63538 LATS2 regulates repressive epigenetic integrity via regulation of Polycomb repressive complex 2
Relations
BioProject PRJNA268339

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE63534_RAW.tar 42.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap