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Series GSE63532 Query DataSets for GSE63532
Status Public on Jul 26, 2016
Title miRNA array profiling of Lats2 deficient mouse embryonic fibroblasts
Organism Mus musculus
Experiment type Non-coding RNA profiling by array
Summary Chromatin modifying activities for construction of appropriate epigenetic landscapes by polycomb repressive complex 2 (PRC2) play an essential role in development and tumorigenesis. However, the spatiotemporal mechanisms by which PRC2 achieves diverse epigenomes for specific tissue or cellular contexts remain poorly understood. Here, we discovered that LATS2 knockout causes dysregulation of PRC2 and subsequent transcriptome changes for differentiation in both mouse and human cells. LATS2 depletion dependent dysregulation of PRC2 also effects H3K4me3 and forms negative feedback loop for maintenance of PRC2. Further analyses reveal that LATS2 on chromatin binds to EZH2 and LATS2 has ability to phosphorylate PRC2 in vitro. These LATS2 dependent H3K27me3 targets are highly induced during neurogenesis, and statistical analysis of glioblastoma multiforme reveals that LATS2-high cases show more dedifferentiated transcriptome and poor prognosis with silencing of H3K27me3 targets. These observations suggest that LATS2-mediated epigenome coordination is pivotal for development and disease, including cancer.
Overall design The rabeled cRNAs from Lats2-/- mouse embryonic fibroblasts (MEFs) and litter wild type MEFs were subjected to Agilent Mouse miRNA Microarray 8 x 15K. Each sample was run in biological dupulicate (different PDL).
Contributor(s) Okuzaki D, Torigata K
Citation(s) 27434182
Submission date Nov 21, 2014
Last update date Jul 26, 2016
Contact name Daisuke Okuzaki
Phone +81-6-6879-4935
Organization name Osaka univ.
Department Immunology Frontier Research Center
Lab Human Immunology (Single Cell Genomics)
Street address Yamadaoka 3-1
City Suita
State/province Osaka
ZIP/Postal code 565-0871
Country Japan
Platforms (1)
GPL10384 Agilent-021828 Unrestricted Mouse miRNA Microarray (V2) (Feature Number version)
Samples (4)
GSM1551875 MEF_Lats2KO_PDL70
GSM1551876 MEF_Lats2KO_PDL96
GSM1551877 MEF_Lats2WT_PDL68
This SubSeries is part of SuperSeries:
GSE63538 LATS2 regulates repressive epigenetic integrity via regulation of Polycomb repressive complex 2
BioProject PRJNA268341

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE63532_RAW.tar 7.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data provided as supplementary file

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