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Series GSE63499 Query DataSets for GSE63499
Status Public on Nov 21, 2014
Title DNA Methylation Changes in Whole Blood and CD16+ Neutrophils in Response to Chronic Folic Acid Supplementation in Women of Childbearing Age
Organism Homo sapiens
Experiment type Methylation profiling by genome tiling array
Summary Folate, a water-soluble vitamin, is a key source of one-carbon groups for DNA methylation, but studies of the DNA methylation response to supplemental folic acid yield inconsistent results. The aim of this study was to determine if DNA methylation patterns in the dominant white blood cell type, neutrophils, would respond differently than whole blood in response to chronic folic acid supplementation.
Saliva and blood methylation was clearly distinguishable though there was positive correlation overall. There was little correlation in CpG sites within relevant candidate genes. Correlated CpG sites were more likely to occur in areas of low CpG density (i.e. CpG shores and open seas). There was more variability in CpG sites from saliva than blood, which may reflect its heterogeneity. Thus, this study provides a framework for using DNA methylation from saliva and suggests that DNA methylation of saliva may offer distinct opportunities for epidemiological and longitudinal studies of psychiatric traits.
Overall design DNA methylation was assessed in whole blood and CD16+ neutrophils from obese and normal weight adult women undergoing 8 weeks of folate supplementation. Blood was collected in EDTA vacuum tubes, and CD16+ nutrophils were isolated by positive selection with Dynabeads. DNA was extracted using the DNeasy Kit (Qiagen). DNA methylation was interrogated for each sample using the HumanMethylation450 BeadChip (Illumina).
Contributor(s) Shade DC, Park HJ, Hausman DB, Hohos N, Meagher RB, Kauwell GP, Lewis RD, Smith AK, Bailey LB
Citation(s) 30499755
Submission date Nov 20, 2014
Last update date Sep 12, 2023
Contact name Alicia Smith
Organization name Emory University
Street address 101 Woodruff Circle
City Atlanta
ZIP/Postal code 30322
Country USA
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (60)
GSM1551105 1003.8wk.CD16
GSM1551106 1003.BL.CD16
GSM1551107 1004.8wk.CD16
BioProject PRJNA268081

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE63499_RAW.tar 183.1 Mb (http)(custom) TAR
GSE63499_unmethylated_methylated_intensities.csv.gz 246.3 Mb (ftp)(http) CSV
Processed data included within Sample table

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