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Series GSE63494 Query DataSets for GSE63494
Status Public on Dec 01, 2016
Title A ChIP-seq spike-in method enables detection of global histone modification changes across the genome
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary This study outlines a method that dramatically alters the interpretation of ChIP-seq data and will improve the quantitative comparison of histone modification maps across biological contexts or across various conditions within a given biological context.
Overall design We introduced a small fraction of Drosophila chromatin into human ChIP samples and added a Drosophila-specific antibody as a means to consistently precipitate Drosophila chromatin as a minor fraction of the total ChIP DNA. The Drosophila ChIP-seq tags are used to normalize the human ChIP-seq data from DMSO and EZH2 inhibitor-treated samples. Employing this strategy, a substantial reduction in H3K27me3 levels is observed across the genome upon EZH2 inhibitor treatment.
Contributor(s) Egan B, Labhart P, Yuan C, Zhao F, Hatton C, Bryant BM, Trojer P
Citation(s) 27875550
Submission date Nov 20, 2014
Last update date May 15, 2019
Contact name Barbara M Bryant
Organization name Constellation Pharmaceuticals
Street address 215 First Street
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (9)
GSM1551046 KARPAS-422_DMSO_8d_H3K9me3
GSM1551047 KARPAS-422_EZH2i_4d_H3K9me3
GSM1551048 KARPAS-422_EZH2i_8d_H3K9me3
BioProject PRJNA268057
SRA SRP050075

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Supplementary file Size Download File type/resource
GSE63494_RAW.tar 1.3 Gb (http)(custom) TAR (of BW, TDF)
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Raw data are available in SRA
Processed data provided as supplementary file

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