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Series GSE63482 Query DataSets for GSE63482
Status Public on Dec 31, 2014
Title Transcriptional Profiling of Sorted Mouse Cortical Projection Neuron Subtypes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Neuronal development requires a complex choreography of transcriptional decisions to obtain specific cellular identities. Realizing the ultimate goal of identifying genome-wide signatures that define and drive specific neuronal fates has been hampered by enormous complexity in both time and space during development. Here, we have paired high-throughput purification of pyramidal neuron subclasses with deep profiling of spatiotemporal transcriptional dynamics during corticogenesis to resolve lineage choice decisions. We identified numerous features ranging from spatial and temporal usage of alternative mRNA isoforms and promoters to a host of mRNA genes modulated during fate specification. Notably, we uncovered numerous long non-coding RNAs with restricted temporal and cell type specific expression. To facilitate future exploration, we provide an interactive online database to enable multidimensional data mining and dissemination. This multi-faceted study generates a powerful resource and informs understanding of the transcriptional regulation underlying pyramidal neuron diversity in the neocortex.
Overall design 24 distinct samples analyzed. 2 replicates each of 3 distinct sorted mouse cortical projection neuron subtypes at 4 developmental time points.
Citation(s) 25556833
Submission date Nov 19, 2014
Last update date May 15, 2019
Contact name Loyal A Goff
Organization name Johns Hopkins University
Department Institute of Genetic Medicine
Lab Goff
Street address 733 N. Broadway Avenue
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (24)
GSM1551750 E15 Sorted callosal projection neurons replicate 1
GSM1551751 E15 Sorted callosal projection neurons replicate 2
GSM1551752 E15 Sorted subcerebral projection neurons replicate 1
BioProject PRJNA268146
SRA SRP050113

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Supplementary file Size Download File type/resource 1.1 Mb (ftp)(http) TXT
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